Upcroft P
Queensland Institute of Medical Research, Brisbane, Queensland, Australia.
Drug Resist Updat. 1998;1(3):166-8. doi: 10.1016/s1368-7646(98)80035-6.
The advantages and limitations of determining mechanisms of drug resistance in Giardia duodenalis laboratory isolates, which have been generated in a number of ways, is weighed against the difficulty of analysing mechanisms in clinical isolates with a large diversity of genetic and expression capabilities. Using isogenic strains to follow changes in enzyme regulation involved in drug resistance, we have been able to assess the full capability of the parasite in developing drug resistance mechanisms. The complementarity of the two approaches, clinical versus laboratory induced drug resistance, and continuing comparison with other organisms, particularly the anaerobic bacteria with which Giardia has strong affiliations, is emphasized. These considerations lead to the study of the population genetics of drug resistance, and strategies critical for rational drug usage, design and therapy.
通过多种方式获得的十二指肠贾第鞭毛虫实验室分离株在确定耐药机制方面的优势和局限性，与分析具有多种遗传和表达能力的临床分离株中耐药机制的难度进行了权衡。利用同基因菌株追踪耐药相关酶调节的变化，我们得以评估寄生虫产生耐药机制的全部能力。强调了两种方法的互补性，即临床诱导耐药与实验室诱导耐药，并持续与其他生物进行比较，特别是与贾第鞭毛虫有密切联系的厌氧菌。这些考量引发了对耐药群体遗传学的研究，以及对合理用药、药物设计和治疗至关重要的策略的研究。
