Wu Ning, Zhou Deng-Feng, Qi Jie-Lin, Zhang Xi-Qin, Bu Bing, Liu Pu-Xia, Wang Ming-Yu, Sun Han-Ying, Liu Wei-Li
Department of Radiation Oncology, Shandong Tumor Hospital and Institute of Oncology, Jinan 250117, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Oct;14(5):1004-7.
This study was purposed to investigate the effect of ligustrazine on the expression of bFGF in bone marrow stromal cells (BMSC) and to explore the mechanism of hematopoietic reconstitution after bone marrow transplantation (BMT). The mice were randomly divided into 3 groups: normal group, saline group and ligustrazine group. BMT mouse models were established. The mice of normal group were not treated, the mice of saline group were given normal saline (0.2 ml/mouse, twice a day) through gastric tube, while the mice of ligustrazine group were given ligustrazine (0.2 ml/mouse, twice a day) through gastric tube. On day 7, 14, 21 and 28 after BMT, the femora were taken and the bone marrow mononuclear cell (BMMNC) suspensions were used for the cultivation of bone marrow stromal cells according to Dexter's culture method. The mRNA and protein expressions of bFGF in BMSC were assayed by RT-PCR and Western blot respectively. The results showed that the expression of bFGF in BMSC on the level of mRNA and protein were all reduced significantly after BMT, and increased slowly with the time. On day 7, 14 and 21 after BMT, the expressions of bFGF mRNA and protein in bone marrow stromal cells of ligustrazine group and saline group were lower than that in bone marrow stromal cells of normal group, but the expressions of bFGF mRNA and protein in ligustrazine group were obviously higher than that in saline group (P < 0.01 or P < 0.05). On day 28 after BMT, the expressions of bFGF mRNA and protein in ligustrazine group returned to normal level, while the expressions of bFGF mRNA and protein in saline group not returned to normal level, there was significant difference between these two groups. It is concluded that ligustrazine can enhance bFGF expression level in bone marrow stromal cells after syngeneic bone marrow transplantation in mice, which confirms that ligustrazine can enhance the repair of bone marrow microvessels, improve bone marrow microenvironment and promote hematopoietic reconstitution.
本研究旨在探讨川芎嗪对骨髓基质细胞（BMSC）中碱性成纤维细胞生长因子（bFGF）表达的影响，并探讨骨髓移植（BMT）后造血重建的机制。将小鼠随机分为3组：正常组、生理盐水组和川芎嗪组。建立BMT小鼠模型。正常组小鼠不做处理，生理盐水组小鼠通过胃管给予生理盐水（0.2 ml/只，每日2次），而川芎嗪组小鼠通过胃管给予川芎嗪（0.2 ml/只，每日2次）。在BMT后第7、14、21和28天，取股骨，采用Dexter培养法将骨髓单个核细胞（BMMNC）悬液用于培养骨髓基质细胞。分别采用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法检测BMSC中bFGF的mRNA和蛋白表达。结果显示，BMT后BMSC中bFGF的mRNA和蛋白水平表达均显著降低，并随时间缓慢升高。在BMT后第7、14和21天，川芎嗪组和生理盐水组骨髓基质细胞中bFGF mRNA和蛋白的表达低于正常组骨髓基质细胞，但川芎嗪组中bFGF mRNA和蛋白的表达明显高于生理盐水组（P<0.01或P<0.05）。在BMT后第28天，川芎嗪组中bFGF mRNA和蛋白的表达恢复至正常水平，而生理盐水组中bFGF mRNA和蛋白的表达未恢复至正常水平，两组间差异有统计学意义。结论：川芎嗪可提高小鼠同基因骨髓移植后骨髓基质细胞中bFGF的表达水平，证实川芎嗪可促进骨髓微血管修复，改善骨髓微环境，促进造血重建。
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