嗜吞噬细胞无形体Ap-变体1株在蜱源细胞系中的分离

Isolation of Anaplasma phagocytophilum strain Ap-variant 1 in a tick-derived cell line.

作者信息

Massung Robert F, Levin Michael L, Munderloh Ulrike G, Silverman David J, Lynch Meghan J, Kurtti Timothy J

机构信息

Centers for Disease Control and Prevention, 1600 Clifton Rd., MS G-13, Atlanta, GA 30333, USA.

出版信息

Ann N Y Acad Sci. 2006 Oct;1078:541-4. doi: 10.1196/annals.1374.105.

DOI:10.1196/annals.1374.105

PMID:17114772

Abstract

Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.

摘要

嗜吞噬细胞无形体Ap-变异体1菌株的十个分离株在来源于肩突硬蜱（肩突硬蜱）的细胞系ISE6中制备。两个分离株从实验室感染的山羊中获得，八个分离株从野外采集的肩突硬蜱中获得。每个分离株的16S rRNA序列与先前描述的Ap-变异体1菌株的序列相同。这些是Ap-变异体1菌株的首批组织培养分离株，将有助于进一步表征该菌株的生物学和抗原特性。