蛋白质折叠液相色谱及其最新进展。
Protein folding liquid chromatography and its recent developments.
作者信息
Geng Xindu, Wang Chaozhan
机构信息
Institute of Modern Separation Science, Key Laboratory of Separation Science in Shaanxi Province, Northwest University, Xi'an 710069, PR China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):69-80. doi: 10.1016/j.jchromb.2006.10.068. Epub 2006 Nov 20.
The ultimate goal of proteomics is to identify biologically active proteins and to produce them using biotechnology tools such as bacterial hosts. However, proteins produced by Escherichia coli must be refolded to their native state. Protein folding liquid chromatography (PFLC) is a new method developed in recent years, and it is widely used in molecular biology and biotechnology. In this paper, the new method, PFLC is introduced and its recent development is reviewed. In addition the paper includes definitions, advantages, principles, applications for both laboratory and large scales, apparatus, and effecting factors of PFLC. In addition, the role of this method in the future is examined.
蛋白质组学的最终目标是鉴定生物活性蛋白，并使用诸如细菌宿主等生物技术工具来生产这些蛋白。然而，大肠杆菌产生的蛋白质必须重新折叠成其天然状态。蛋白质折叠液相色谱法（PFLC）是近年来开发的一种新方法，它在分子生物学和生物技术中得到了广泛应用。本文介绍了PFLC这种新方法，并综述了其最新进展。此外，本文还包括PFLC的定义、优点、原理、实验室和大规模应用、仪器设备以及影响因素。此外，还探讨了该方法在未来的作用。
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