Jordan B R, Chow W S, Strid A, Anderson J M
CSIRO, Division of Plant Industry, Canberra ACT, Australia.
FEBS Lett. 1991 Jun 17;284(1):5-8. doi: 10.1016/0014-5793(91)80748-r.
The cab and psb A RNA transcript levels have been determined in Pisum sativum leaves exposed to supplementary ultraviolet-B radiation. The nuclear-encoded cab transcripts are reduced to low levels after only 4 h of UV-B treatment and are undetectable after 3 days exposure. In contrast, the chloroplast-encoded psb A transcript levels, although reduced, are present for at least 3 days. After short periods of UV-B exposure (4 h or 8 h), followed by recovery under control conditions, cab RNA transcript levels had not recovered after 1 day, but were re-established to ca. 60% of control levels after 2 more days. Increased irradiance during exposure to UV-B reduced the effect upon cab transcripts, although the decrease was still substantial. These results indicate rapid changes in the cellular regulation of gene expression in response to supplementary UV-B and suggest increased UV-B radiation may have profound consequences for future productivity of sensitive crop species.
已测定了暴露于补充紫外线B辐射下的豌豆叶片中cab和psb A RNA转录水平。核编码的cab转录本在紫外线B处理仅4小时后就降至低水平，照射3天后无法检测到。相比之下，叶绿体编码的psb A转录本水平虽然降低，但至少存在3天。在短时间暴露于紫外线B（4小时或8小时）后，接着在对照条件下恢复，cab RNA转录本水平在1天后未恢复，但再过2天后恢复到对照水平的约60%。在暴露于紫外线B期间增加辐照度可降低对cab转录本的影响，尽管降低幅度仍然很大。这些结果表明，响应补充紫外线B，基因表达的细胞调控发生了快速变化，并表明增加的紫外线B辐射可能对敏感作物品种的未来生产力产生深远影响。
