[纸片扩散法与聚合酶链反应检测金黄色葡萄球菌菌株耐甲氧西林情况的比较]

[Comparison of disk-diffusion method and PCR for detection of methicillin resistance in Staphylococcus aureus strains].

作者信息

Kaczmarek Agnieszka, Budzyńska Anna, Mikołajczyk Dorota, Gospodarek Eugenia

机构信息

Katedra i Zaklad Mikrobiologii, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytetu Mikolaja Kopernika w Tomninu.

出版信息

Med Dosw Mikrobiol. 2006;58(2):87-93.

PMID:17133901

Abstract

The aim of the study was to compare the disk-diffusion (oxacillin 1 microg, cefoxitin 30 microg) method and PCR for detection of methicillin-resistance in S. aureus. The investigation were carried out on 120 S. aureus strains isolated from clinical materials of patients hospitalized in the University Hospital at the L. Rydygier Collegium Medicum in Bydgoszcz, University of Nicolaus Copernicus in Toruń. Of the 120 S. aureus strains tested, 60 (50%) were mecA-positive by PCR. Consistency of results between oxacillin disk-difussion method and PCR amounted 92.5% and cefoxitin disk-diffusion method and PCR--98.3%. The oxacillin disk-difussion method falsely identified 3 (2.5%) strains as MSSA (sensitivity 95.0%) and 4 strains as MRSA (specificity 93.3%) in comparison with PCR. The cefoxitin disk-diffusion method falsely identified 2 (1.6%) strains as MSSA (sensitivity 96.7%) and there were no false resistant results (specificity 100%). Our results showed that in disk-diffusion tests, cefoxitin is a better than oxacillin for the identification of MRSA.

摘要

本研究旨在比较纸片扩散法（苯唑西林1微克、头孢西丁30微克）和聚合酶链反应（PCR）检测金黄色葡萄球菌耐甲氧西林情况。研究对象为从比得哥什的里迪吉尔医学院大学医院、托伦的哥白尼大学医院住院患者临床材料中分离出的120株金黄色葡萄球菌。在检测的120株金黄色葡萄球菌中，PCR检测显示60株（50%）mecA基因阳性。苯唑西林纸片扩散法与PCR结果的一致性为92.5%，头孢西丁纸片扩散法与PCR结果的一致性为98.3%。与PCR相比，苯唑西林纸片扩散法将3株（2.5%）菌株错误鉴定为甲氧西林敏感金黄色葡萄球菌（MSSA，敏感性95.0%），4株错误鉴定为耐甲氧西林金黄色葡萄球菌（MRSA，特异性93.3%）。头孢西丁纸片扩散法将2株（1.6%）菌株错误鉴定为MSSA（敏感性96.7%），且无假耐药结果（特异性100%）。我们的结果表明，在纸片扩散试验中，头孢西丁在鉴定MRSA方面优于苯唑西林。