Mohamed A, Jansson C
Department of Biochemistry, Arrhenius Laboratories, Stockholm University, Sweden.
Plant Mol Biol. 1991 May;16(5):891-7. doi: 10.1007/BF00015080.
Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression in Synechocystis 6803. The production of psbA transcripts was stimulated by light in a process that operated independently of the photosynthetic electron transport. However, stability of the psbA transcript increased in the dark and was controlled by photosynthetic electron transport. The psbA transcript was remarkably stable in the dark, with a half-life of approximately 7 hours. By contrast, the regulatory pattern for the rbcL-S genes was quite different. The light-stimulated production of rbcL-S transcripts was dependent on an intact photosynthetic electron transport, and rbcL-S transcript stability was higher under illuminated conditions than in darkness.
在存在和不存在光合作用抑制剂二氯苯基二甲基脲（DCMU）和甲基紫精的情况下，跟踪了聚球藻6803中psbA和rbcL-S转录本的积累和稳定性。我们的结果表明，转录本产生和转录本稳定性都是聚球藻6803中psbA基因表达的重要调控元件。psbA转录本的产生在一个独立于光合电子传递的过程中受到光的刺激。然而，psbA转录本的稳定性在黑暗中增加，并受光合电子传递的控制。psbA转录本在黑暗中非常稳定，半衰期约为7小时。相比之下，rbcL-S基因的调控模式则大不相同。rbcL-S转录本受光刺激的产生依赖于完整的光合电子传递，并且rbcL-S转录本在光照条件下的稳定性高于黑暗中。
