Reedy E A, Cottrell J R, Resau J H
Department of Pathology, University of Maryland School of Medicine, Baltimore.
Pathobiology. 1991;59(2):62-8. doi: 10.1159/000163618.
Flow cytometry analysis of primary bladder carcinoma cells is appropriate for demonstrating the association between the presence of aneuploid cells and increased malignant potential for transitional cell carcinoma (TCC). Our laboratory previously has reported a specific alteration in the cytokeratin (CK) pattern of experimentally induced bladder carcinomas in rats. Unique immunohistochemically detectable changes in CK expression were the loss of CK 13 expression in invasive TCC cells and the loss of CK 19 expression in induced TCC. We now report the correlation of these changes in CK expression with cellular aneuploidy of the induced bladder carcinomas. Bladder carcinomas were induced in rats by direct instillation of N-methyl-N-nitrosourea. Immunohistochemistry was performed on the induced tumors using four commercially available monoclonal antibodies specific for CK 18 (TR1031), CK 13 (K8.12), CK 19 (K4.62), and the CKs 5, 7, and 8 (K8.13). Flow cytometry data from the induced bladder carcinomas was analyzed and the DNA index, proliferative index, and percent aneuploid cells were calculated for each time point. The percent aneuploid cells and CK 19 staining were tested statistically and were shown to be negatively correlated. We therefore hypothesize that the combination of the loss of CK 19 as detected by the antibody K4.62 coupled with the presence of aneuploid cells in histopathologically diagnosed invasive TCC is a significant factor in predicting the prognosis for any given diagnosis.
原发性膀胱癌细胞的流式细胞术分析适用于证明非整倍体细胞的存在与移行细胞癌（TCC）恶性潜能增加之间的关联。我们实验室此前报告了大鼠实验性诱导膀胱癌中细胞角蛋白（CK）模式的特定改变。CK表达中独特的免疫组化可检测变化是浸润性TCC细胞中CK 13表达缺失以及诱导性TCC中CK 19表达缺失。我们现在报告这些CK表达变化与诱导性膀胱癌细胞非整倍体的相关性。通过直接滴注N-甲基-N-亚硝基脲在大鼠中诱导膀胱癌。使用四种针对CK 18（TR1031）、CK 13（K8.12）、CK 19（K4.62）以及CKs 5、7和8（K8.13）的市售单克隆抗体对诱导的肿瘤进行免疫组化。分析诱导性膀胱癌的流式细胞术数据，并计算每个时间点的DNA指数、增殖指数和非整倍体细胞百分比。对非整倍体细胞百分比和CK 19染色进行统计学检验，结果显示呈负相关。因此，我们假设通过抗体K4.62检测到的CK 19缺失与组织病理学诊断的浸润性TCC中非整倍体细胞的存在相结合是预测任何给定诊断预后的一个重要因素。
