Akutsu Hidenori, Cowan Chad A, Melton Douglas
Harvard University, Howard Huges Medical Institute, Cambridge, Massachusetts, USA.
Methods Enzymol. 2006;418:78-92. doi: 10.1016/S0076-6879(06)18005-2.
Human embryonic stem cells hold great promise in furthering our treatment of disease and increasing our understanding of early development. This chapter describes protocols for the derivation and maintenance of human embryonic stem cells. In addition, it summarizes briefly several alternative methods for the culture of human embryonic stem cells. Thus, this chapter provides a good starting point for researchers interested in harnessing the potential of human embryonic stem cells.
人类胚胎干细胞在推动疾病治疗和增进我们对早期发育的理解方面具有巨大潜力。本章描述了人类胚胎干细胞的分离和维持方案。此外,还简要总结了几种培养人类胚胎干细胞的替代方法。因此,本章为对利用人类胚胎干细胞潜力感兴趣的研究人员提供了一个良好的起点。