人类胚胎干细胞中人类基因组的操控。

Manipulation of the human genome in human embryonic stem cells.

作者信息

Kopper Oded, Benvenisty Nissim

机构信息

Department of Genetics, Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.

出版信息

Stem Cell Rev. 2005;1(2):145-50. doi: 10.1385/SCR:1:2:145.

DOI:10.1385/SCR:1:2:145

PMID:17142849

Abstract

Human embryonic stem cells (HESCs) have a tremendous clinical and scientific importance since they may serve as a cell source for transplantation and as a system for the study of human development and disease. The genetic engineering of HESCs has become instrumental in achieving these goals. Here we discuss various methodologies to genetically manipulate HESCs and propose a variety of applications of the modified cells in basic and applied research.

摘要

人类胚胎干细胞（HESCs）具有巨大的临床和科学重要性，因为它们可作为移植的细胞来源以及用于研究人类发育和疾病的系统。对HESCs进行基因工程已成为实现这些目标的重要手段。在此，我们讨论对HESCs进行基因操作的各种方法，并提出经修饰细胞在基础研究和应用研究中的多种应用。