人胶质瘤中PDCD4表达频繁缺失：其在胶质瘤发生中的可能作用

Frequent loss of PDCD4 expression in human glioma: possible role in the tumorigenesis of glioma.

作者信息

Gao Fei, Zhang Pin, Zhou Chuixian, Li Jianfeng, Wang Qun, Zhu Faliang, Ma Chunhong, Sun Wensheng, Zhang Lining

机构信息

Institute of Immunology, School of Medicine, QiLu Hospital, Shangdong University, Shandong, 44 Wenhua Xilu, Jinan 250021, P.R. China.

出版信息

Oncol Rep. 2007 Jan;17(1):123-8.

PMID:17143488

Abstract

Programmed cell death 4 (PDCD4) was recently identified as a novel tumor suppressor gene. The loss of PDCD4 expression was found in several types of human cancer cell lines. To date, however, the status of PDCD4 expression in human glioma tissue is not known. In the present study, the expression of PDCD4 in 30 glioma samples was determined at both mRNA and protein levels by means of RT-PCR, Western blotting, and immunohistochemistry. Herein, we demonstrate, for the first time, that 47% (14/30) of glioma samples lost the expression of PDCD4 mRNA, and 77% (23/30) of glioma samples lacked the PDCD4 protein expression, whereas adjacent normal glial tissues expressed high levels of PDCD4 mRNA and protein. Furthermore, the loss of PDCD4 expression does not significantly correlate with the pathological and clinical features of the glioma. Our new data suggest that the loss of PDCD4 expression is a frequent event in human glioma and may partially contribute to the development of the tumor.

摘要

程序性细胞死亡4（PDCD4）最近被鉴定为一种新的肿瘤抑制基因。在几种人类癌细胞系中发现了PDCD4表达缺失。然而，迄今为止，人类胶质瘤组织中PDCD4的表达情况尚不清楚。在本研究中，通过逆转录聚合酶链反应（RT-PCR）、蛋白质印迹法和免疫组织化学方法，在mRNA和蛋白质水平上测定了30例胶质瘤样本中PDCD4的表达。在此，我们首次证明，47%（14/30）的胶质瘤样本失去了PDCD4 mRNA的表达，77%（23/30）的胶质瘤样本缺乏PDCD4蛋白表达，而相邻的正常神经胶质组织则表达高水平的PDCD4 mRNA和蛋白。此外，PDCD4表达缺失与胶质瘤的病理和临床特征无显著相关性。我们的新数据表明，PDCD4表达缺失在人类胶质瘤中是常见事件，可能部分促成了肿瘤的发生。