Risvanli Ali, Timurkan Huseyin, Akpolat Nusret, Gulacti Irem, Ulakoglu Erdal
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey.
J Assist Reprod Genet. 2006 Nov-Dec;23(11-12):401-6. doi: 10.1007/s10815-006-9078-5. Epub 2006 Dec 2.
The study aimed to determine effects of FSH applications on follicle survival, development and hormone output and antigenicity of rat ovarian tissue autografts placed at subcutaneous or subperitoneal sites.
A total of sixteen female rats were used in the study. The animals were divided into three groups. Ovaries were dissected and then transplanted under the peritoneum in the first group animals (n = 5) or under the skin in the second group animals (n = 6). And the animals in the third group (n = 5) were sham operated. Following operations, intramuscular injection of 8 IU of rhFSH were made daily to the animals in first and second groups from the first day of operation through thirty days. Vaginal irrigation samples were prepared daily from the animals for 30 days. The concentrations of serum estradiol and antiovarian antibodies in the blood were determined using ELISA on the last day of vaginal irrigations. Histopathological examination of the ovaries that were transplanted was made.
Results showed that cyclic variations were noticed in the samples of vaginal irrigation by day 30 in the animals of first and second groups. However, no significant differences were seen between groups. The concentration of blood serum estradiol was higher in the animals of first group. Decrease in numbers of primary follicles were found in the animals of second group and lesser corpus luteum were found in the animals of control group on the histopathological examinations of transplanted ovaries. All rats in the first and second groups were defined as seropositive for antiovarian antibodies. When the OD values were compared between first and second groups, it was identified that the OD values of rats in the first group was higher than it was seen in the second group.
The ovarian transplantation without vascular pedicle in rats is characterized by follicular hyperplasia endocrinologically functional. Being seropositive of all rats in first and second groups in terms of antiovarian antibodies is an indicator to these antibodies does not affect the functions of transplanted ovaries. It is believed that the highness of OD values in the group which is transplanted beneath the peritoneum is based on the highness of estradiol concentrations in these animals.
本研究旨在确定促卵泡激素（FSH）应用对皮下或腹膜内植入的大鼠卵巢组织自体移植物的卵泡存活、发育、激素分泌及抗原性的影响。
本研究共使用16只雌性大鼠。将动物分为三组。第一组动物（n = 5）的卵巢被解剖后移植到腹膜下，第二组动物（n = 6）的卵巢被解剖后移植到皮下。第三组动物（n = 5）进行假手术。术后，第一组和第二组动物从手术第一天起至第30天每天肌肉注射8 IU重组人促卵泡激素（rhFSH）。连续30天每天采集动物的阴道冲洗样本。在阴道冲洗的最后一天，使用酶联免疫吸附测定法（ELISA）测定血液中血清雌二醇和抗卵巢抗体的浓度。对移植的卵巢进行组织病理学检查。
结果显示，第一组和第二组动物在第30天时阴道冲洗样本出现周期性变化。然而，各组之间未观察到显著差异。第一组动物血清雌二醇浓度较高。在移植卵巢的组织病理学检查中，第二组动物的初级卵泡数量减少，对照组动物的黄体较少。第一组和第二组的所有大鼠均被定义为抗卵巢抗体血清阳性。比较第一组和第二组的光密度（OD）值时，发现第一组大鼠的OD值高于第二组。
大鼠无血管蒂卵巢移植具有卵泡增生且内分泌功能正常的特点。第一组和第二组所有大鼠抗卵巢抗体血清阳性表明这些抗体不影响移植卵巢的功能。据信，腹膜下移植组OD值较高是基于这些动物中雌二醇浓度较高。
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