5-氨基咪唑-4-甲酰胺核苷对新生链脲佐菌素诱导的糖尿病大鼠胰腺的影响。

The effects of 5-aminoimidazole-4-carboxamide riboside on the pancreas in neonatal streptozotocin-diabetic rats.

作者信息

Ozturk M, Bolkent S, Kaya-Dagistanli F, Tuncdemir M, Yilmazer S, Akkan A G

机构信息

Department of Medical Biology, Cerrahpasa Faculty of Medicine, Istanbul University, Cerrahpasa, Istanbul, Turkey.

出版信息

Acta Diabetol. 2006 Nov;43(3):61-5. doi: 10.1007/s00592-006-0214-6.

DOI:10.1007/s00592-006-0214-6

PMID:17143782

Abstract

In this study, we aimed to determine the alterations of beta-cell ultrastructure, insulin mRNA and protein products of the same gene on the pancreas of rats following long-term treatment of 5-aminoimidazole-4-carboxamide riboside (AICAR). A single dose of streptozotocin (STZ) 100 mg/kg was injected intraperitoneally (i.p.) to 2-day-old newborn (n2) rats. The rats were divided into three groups. The first group was the n2 STZ-diabetic rats. The second group consisted of n2 STZ-diabetic rats treated with AICAR 10 mg/kg/day for one month. The third group was non-diabetic control rats. Our findings demonstrate that AICAR treatment decreases the blood glucose level but increases the body weight in n2 STZ-diabetic rats. In the AICAR-treated group, numerous beta cells showed increased insulin gene expression. We also observed increased exocytosis in this group, in an ultrastructural manner. As a result, it is suggested that AICAR may induce insulin synthesis and betacell regeneration in n2 STZ-diabetic rats.

摘要

在本研究中，我们旨在确定长期给予5-氨基咪唑-4-甲酰胺核苷（AICAR）后，大鼠胰腺中β细胞超微结构、胰岛素mRNA以及同一基因的蛋白质产物的变化。将100 mg/kg的单剂量链脲佐菌素（STZ）腹腔注射给2日龄新生（n2）大鼠。大鼠被分为三组。第一组是n2 STZ糖尿病大鼠。第二组由用10 mg/kg/天的AICAR治疗一个月的n2 STZ糖尿病大鼠组成。第三组是非糖尿病对照大鼠。我们的研究结果表明，AICAR治疗可降低n2 STZ糖尿病大鼠的血糖水平，但增加其体重。在AICAR治疗组中，许多β细胞显示胰岛素基因表达增加。我们还以超微结构的方式观察到该组中胞吐作用增加。因此，提示AICAR可能诱导n2 STZ糖尿病大鼠的胰岛素合成和β细胞再生。