Stoll J, Goldman D
Laboratory of Clinical Studies, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
J Neurosci Res. 1991 Apr;28(4):457-65. doi: 10.1002/jnr.490280402.
The mouse tryptophan hydroxylase gene was isolated and its intron/exon boundaries and putative regulatory sequences identified. To isolate the gene a mouse mastocytoma cDNA clone encoding tryptophan hydroxylase was used to identify and isolate ten overlapping DNA fragments from a mouse genomic library. Restriction mapping and sequence analysis of the clones revealed that the gene contains 11 exons and covers a region of DNA of approximately 21 kb. The transcription initiation site was mapped and the major site of initiation yields an untranslated leader sequence of 124 nucleotides. A minor initiation site is located 9 nucleotides 3' of the major site. The 5' untranslated sequence is interrupted by the first intron. Analysis of the sequence upstream of the initiation site showed the presence of several putative promoter and regulatory sequences. Nine of the ten intron/exon boundaries of tryptophan hydroxylase are conserved with tyrosine hydroxylase and phenylalanine hydroxylase, further delineating the evolutionary relationship of these three genes.
小鼠色氨酸羟化酶基因被分离出来，并确定了其内含子/外显子边界以及假定的调控序列。为了分离该基因，使用了一个编码色氨酸羟化酶的小鼠肥大细胞瘤cDNA克隆，从一个小鼠基因组文库中鉴定并分离出10个重叠的DNA片段。对这些克隆进行限制性图谱分析和序列分析表明，该基因包含11个外显子，覆盖约21kb的DNA区域。确定了转录起始位点，主要起始位点产生一个124个核苷酸的非翻译前导序列。一个次要起始位点位于主要位点下游9个核苷酸处。5'非翻译序列被第一个内含子打断。对起始位点上游序列的分析表明存在几个假定的启动子和调控序列。色氨酸羟化酶的10个内含子/外显子边界中有9个与酪氨酸羟化酶和苯丙氨酸羟化酶保守，进一步描绘了这三个基因的进化关系。
