使用金刚烷基萘二亚胺与二茂铁基-β-环糊精结合进行电化学基因检测。

Electrochemical gene detection by using adamantyl naphthalene diimide coupled with ferrocenyl-beta-cyclodextrin.

作者信息

Sato Shinobu, Nojima Takahiko, Kondo Hiroki, Takenaka Shigeori

机构信息

Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):103-4. doi: 10.1093/nass/48.1.103.

DOI:10.1093/nass/48.1.103

PMID:17150499

Abstract

The adamantyl moieties of adamantylnaphthalene diimide (AND) can be incorporated into the cavity of ferrocenyl-beta-cyclodextrin even where they are bound to double stranded DNA by threading intercalation. When this ternary complex is formed on the DNA probe-immobilized electrode, electrochemical DNA detection is feasible: the electrode hybridized with target DNA gave rise to a current peak corresponding to the ferrocene oxidation upon treatment with AND and ferrocenyl-beta-cyclodextrin (Fc-beta-CD).

摘要

即使金刚烷基萘二亚胺（AND）的金刚烷基部分通过穿插入作用与双链DNA结合，它们也可以被纳入二茂铁基-β-环糊精的腔内。当这种三元复合物在固定有DNA探针的电极上形成时，电化学DNA检测是可行的：与靶DNA杂交的电极在用AND和二茂铁基-β-环糊精（Fc-β-CD）处理后产生了一个对应于二茂铁氧化的电流峰。