反式翻译相关因子的功能分析

Functional analysis of factors involved in trans-translation.

作者信息

Takada Kazuma, Takemoto Chie, Kawazoe Masahito, Konno Takayuki, Matsuta Noriko, Kurita Daisuke, Asano Krisana, Shirouzu Mikako, Yokoyama Shigeyuki, Muto Akira, Himeno Hyouta

机构信息

Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):101-2. doi: 10.1093/nass/49.1.101.

DOI:10.1093/nass/49.1.101

PMID:17150653

Abstract

trans-translation is a rescue system for stalled ribosomes to terminate the translation for recycling of ribosome and lead the incomplete protein on the stalled ribosome to degradation. In trans-translation, several proteins and RNAs are involved. tmRNA and SmpB are only factors known to be essential for trans-translation. EF-Tu and EF-G functions in translation and also are seemed to function in trans-translation. Ribosomal protein S1 is known to bind tmRNA. In this study, we cloned the gene of tmRNA from Thermus thermophilus and analyzed the functions of factors involved in trans-translation in vitro.

摘要

反式翻译是一种针对停滞核糖体的拯救系统，用于终止翻译以便核糖体循环利用，并使停滞核糖体上的不完整蛋白质降解。在反式翻译过程中，涉及多种蛋白质和RNA。tmRNA和SmpB是已知对反式翻译必不可少的唯一因子。EF-Tu和EF-G在翻译中发挥作用，似乎也在反式翻译中发挥作用。已知核糖体蛋白S1可结合tmRNA。在本研究中，我们从嗜热栖热菌中克隆了tmRNA基因，并在体外分析了参与反式翻译的因子的功能。