使用二茂铁基萘二酰亚胺对端粒四链体DNA进行电化学检测。

Electrochemical detection of telomeric quadruplex DNA using ferrocenyl naphthalene diimide.

作者信息

Sato Shinobu, Kondo Hiroki, Nojima Takahiko, Takenaka Shigeori

机构信息

Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka 820-8502, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):237-8. doi: 10.1093/nass/49.1.237.

DOI:10.1093/nass/49.1.237

PMID:17150721

Abstract

Ferrocenylnaphthalene diimide (FND) could strongly bind to the synthetic telomere DNA, 5'-CAT GGT GGT TTG GGT TAG GGT TAG GGT TAG GGT TAC CAC-3', at 0.1 M KCl. The binding affinity of FND for the telomere DNA was ten times higher than that for calf thymus DNA under the conditions employed. Circular dichroism spectra suggested that the telomere DNA can form a tetraplex structure and FND can bind to it. Oligonucleotide extended by telomerase on the electrode could also be detected electrochemically by using FND, thereby suggesting the possibility that telomerase activity may be detected electrochemically.

摘要

二茂铁基萘二亚胺（FND）在0.1M氯化钾条件下能与合成端粒DNA（5'-CAT GGT GGT TTG GGT TAG GGT TAG GGT TAG GGT TAC CAC-3'）强烈结合。在所采用的条件下，FND对端粒DNA的结合亲和力比对小牛胸腺DNA的结合亲和力高十倍。圆二色光谱表明端粒DNA能形成四链体结构，且FND能与之结合。利用FND还能通过电化学方法检测电极上由端粒酶延伸的寡核苷酸，从而表明端粒酶活性有可能通过电化学方法检测。