Yoshimura Takeo, Nishida Kunitada, Uchibayashi Keiichi, Ohuchi Shokichi
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Wakamatsu, Kitakyushu, Fukuoka 808-0196, Japan.
Nucleic Acids Symp Ser (Oxf). 2006(50):305-6. doi: 10.1093/nass/nrl152.
The Rolling Circle Amplification is an effective method of DNA amplification having tandem repeated sequences. Firstly, the template sequence is designed to form a circular structure by the annealing with the primer. The thermostable polymerase is catalyzed to primer and circular template DNA. As a result, tandem repetitive olignucleotide of circular template sequence can be obtained. In our recent study, Bst DNA polymerase catalyzed RCA reaction had been elucidated that the oligonucleotides with tandem repeated sequence were obtained as the ladder band and amplified the enough amounts of DNA within short time. On the other hand, we also focused on the microwave technology for advance to the various chemical and biological reactions. In this study, the microwave was irradiated to RCA reaction on controlling the temperature.
滚环扩增是一种用于扩增具有串联重复序列的DNA的有效方法。首先，通过与引物退火将模板序列设计成环状结构。热稳定聚合酶催化引物与环状模板DNA反应。结果，可以获得环状模板序列的串联重复寡核苷酸。在我们最近的研究中，已经阐明了Bst DNA聚合酶催化的滚环扩增反应，即获得了具有串联重复序列的寡核苷酸作为梯状条带，并在短时间内扩增出了足够量的DNA。另一方面，我们也关注微波技术在促进各种化学和生物反应方面的应用。在本研究中，在控制温度的条件下对滚环扩增反应进行微波辐照。
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