肌醇四磷酸作为第二信使在非洲爪蟾卵母细胞中诱导钙依赖的氯电流。

Inositol tetrakisphosphates as second messengers induce Ca(++)-dependent chloride currents in Xenopus laevis oocytes.

作者信息

Guse A H, Gercken G, Boysen H, Schwarz J R, Meyerhof W

机构信息

Institut für Biochemie und Lebensmittelchemie, Abt. für Biochemie, Universität, Hamburg, FRG.

出版信息

Biochem Biophys Res Commun. 1991 Aug 30;179(1):641-7. doi: 10.1016/0006-291x(91)91420-h.

DOI:10.1016/0006-291x(91)91420-h

PMID:1715700

Abstract

Microinjection of inositol 1,3,4,5-tetrakisphosphate or inositol 1,4,5-trisphosphate induced distinct chloride membrane currents in defolliculated Xenopus laevis oocytes. To decide whether these Cl(-)-currents were due to the injected compounds or their metabolic products, [3H]Ins(1,3,4,5)P4 or [3H]Ins(1,4,5)P3 were injected into oocytes and their metabolites were analyzed by HPLC. Our results indicate that Ins(1,3,4,5)P4 itself or its metabolite Ins(1,3,4,6)P4 is able to induce Cl(-)-membrane currents, most likely by increasing the cytosolic Ca(++)-concentration.

摘要

向去滤泡的非洲爪蟾卵母细胞中微量注射肌醇1,3,4,5-四磷酸或肌醇1,4,5-三磷酸可诱导出不同的氯化物膜电流。为了确定这些Cl⁻电流是由注射的化合物还是其代谢产物引起的，将[³H]肌醇1,3,4,5-四磷酸或[³H]肌醇1,4,5-三磷酸注射到卵母细胞中，并通过高效液相色谱法分析其代谢产物。我们的结果表明，肌醇1,3,4,5-四磷酸本身或其代谢产物肌醇1,3,4,6-四磷酸能够诱导Cl⁻膜电流，最有可能是通过增加胞质Ca²⁺浓度来实现的。