Guse A H, Gercken G, Boysen H, Schwarz J R, Meyerhof W
Institut für Biochemie und Lebensmittelchemie, Abt. für Biochemie, Universität, Hamburg, FRG.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):641-7. doi: 10.1016/0006-291x(91)91420-h.
Microinjection of inositol 1,3,4,5-tetrakisphosphate or inositol 1,4,5-trisphosphate induced distinct chloride membrane currents in defolliculated Xenopus laevis oocytes. To decide whether these Cl(-)-currents were due to the injected compounds or their metabolic products, [3H]Ins(1,3,4,5)P4 or [3H]Ins(1,4,5)P3 were injected into oocytes and their metabolites were analyzed by HPLC. Our results indicate that Ins(1,3,4,5)P4 itself or its metabolite Ins(1,3,4,6)P4 is able to induce Cl(-)-membrane currents, most likely by increasing the cytosolic Ca(++)-concentration.
向去滤泡的非洲爪蟾卵母细胞中微量注射肌醇1,3,4,5-四磷酸或肌醇1,4,5-三磷酸可诱导出不同的氯化物膜电流。为了确定这些Cl⁻电流是由注射的化合物还是其代谢产物引起的，将[³H]肌醇1,3,4,5-四磷酸或[³H]肌醇1,4,5-三磷酸注射到卵母细胞中，并通过高效液相色谱法分析其代谢产物。我们的结果表明，肌醇1,3,4,5-四磷酸本身或其代谢产物肌醇1,3,4,6-四磷酸能够诱导Cl⁻膜电流，最有可能是通过增加胞质Ca²⁺浓度来实现的。
