通过液相色谱和质谱法分析与DNA结合的晚期糖基化终产物

Analysis of DNA-bound advanced glycation end-products by LC and mass spectrometry.

作者信息

Bidmon Clemens, Frischmann Matthias, Pischetsrieder Monika

机构信息

Institute of Pharmacy and Food Chemistry, University of Erlangen-Nuremberg, Erlangen, Germany.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug;855(1):51-8. doi: 10.1016/j.jchromb.2006.11.033. Epub 2006 Dec 11.

DOI:10.1016/j.jchromb.2006.11.033

PMID:17161667

Abstract

Sugars and sugar degradation products readily react in vitro with guanine derivatives, resulting in the formation of DNA-bound advanced glycation end-products (DNA-AGEs). The two diastereomers of N(2)-(1-carboxyethyl)-2'-deoxyguanosine (CEdG(A,B)) and the cyclic adduct of methylglyoxal and 2'-deoxyguanosine (mdG) (N(2)-7-bis(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine have also been detected in cultured cells and/or in vivo. LC-MS/MS methods have been developed to analyze sensitively DNA adducts in vitro and in vivo. In this paper, the chemical structures of possible DNA-AGEs and the application of LC-MS/MS to measure DNA-AGEs are reviewed.

摘要

糖类及糖降解产物在体外易与鸟嘌呤衍生物发生反应，导致形成与DNA结合的晚期糖基化终产物（DNA-AGEs）。N(2)-(1-羧乙基)-2'-脱氧鸟苷（CEdG(A,B)）的两种非对映异构体以及甲基乙二醛与2'-脱氧鸟苷的环状加合物（mdG）（N(2)-7-双(1-羟基-2-氧代丙基)-2'-脱氧鸟苷）也已在培养细胞和/或体内检测到。液相色谱-串联质谱（LC-MS/MS）方法已被开发用于灵敏分析体外和体内的DNA加合物。本文对可能的DNA-AGEs的化学结构以及LC-MS/MS在测量DNA-AGEs中的应用进行了综述。