甲状腺相关性眼病中人类眼眶前脂肪细胞的培养与分化
Culture and differentiation of human orbital preadipocytes in thyroid-associated ophthalmopathy.
作者信息
Wang Lifei, Wu Zhongyao, Yan Jianhua, Yang Huasheng, Chen Zhicong
机构信息
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
出版信息
Yan Ke Xue Bao. 2005 Mar;21(1):27-30, 37.
PURPOSE
To identify whether there are preadipocytes in the orbits of thyroid-associated (TAO) patients and whether they possess the capacity to undergo ophthalmopathy adipocytic differentiation.
METHODS
Orbital fat tissue from TAO patients was placed in primary culture and proliferating cells were sub-passaged. The preadiocytes were identified with immunohistochemistry. Confluent preadipocytes were subjected to a differentiation protocol. And Peroxisome proliferators-associated receptory (PPARgamma) transcript levels were determined before and after differentiation.
RESULT
In primary culture, cells were spindly and showed positive immunostaining for preadipocyte factor-1 (pref-1) protein. During the process of differentiation, orbital cells showed evidence of adipogenesis and relatively greater PPARy gene expression with differentiation.
CONCLUSION
There are active preadipocytes in the orbital tissue of TAO patients which might be able to undergo adipocyte differentiation. These observations suggest that the preadipocytes differentiation contributed to excess orbital adipose tissue volume in TAO.
目的
确定甲状腺相关性眼病（TAO）患者眼眶中是否存在前脂肪细胞，以及它们是否具有发生眼病脂肪细胞分化的能力。
方法
将TAO患者的眼眶脂肪组织进行原代培养，并对增殖细胞进行传代培养。用免疫组织化学法鉴定前脂肪细胞。对汇合的前脂肪细胞进行分化实验。并在分化前后测定过氧化物酶体增殖物激活受体（PPARγ）转录水平。
结果
在原代培养中，细胞呈纺锤形，对前脂肪细胞因子-1（Pref-1）蛋白呈阳性免疫染色。在分化过程中，眼眶细胞显示出脂肪生成的证据，并且随着分化PPARγ基因表达相对增加。
结论
TAO患者眼眶组织中存在活跃的前脂肪细胞，它们可能能够进行脂肪细胞分化。这些观察结果表明，前脂肪细胞分化导致TAO患者眼眶脂肪组织体积过多。
Zotero 插件