共轭亚油酸对人巨噬细胞中磷脂酶A(2)活性的抑制作用。
Inhibition of phospholipase A(2) activity by conjugated linoleic acids in human macrophages.
作者信息
Stachowska Ewa, Dziedziejko Violetta, Safranow Krzysztof, Gutowska Izabela, Adler Grazyna, Ciechanowicz Andrzej, Machaliński Bogusław, Chlubek Dariusz
机构信息
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, al. Powstancow Wlkp. 72, 70-111 Szczecin, Poland.
出版信息
Eur J Nutr. 2007 Feb;46(1):28-33. doi: 10.1007/s00394-006-0626-7. Epub 2006 Dec 11.
The objective of this study was to assess the effect of conjugated linoleic acid isomers (CLAs) on the expression and activity of phospholipases A(2) (PLA(2)) in human macrophages. Macrophages were incubated with 30 microM cis-9, trans-11 and trans-10, cis-12 CLAs for 48 h. After incubation, the total activity of phospholipases as well as the expression of mRNA for cytosolic (cPLA(2)) and secretory (sPLA(2)) phospholipases and activity of sPLA(2) were measured. Both CLA isomers reduced the total activity of PLA(2) (by 30.2%, P < 0.01 for cis-9, trans-11 CLA and by 30%, P < 0.001 for trans-10, cis-12 CLA). Trans-10, cis-12 CLA isomer downregulated the expression of mRNA of sPLA(2) and decreased the enzymatic activity of this enzyme (by 23%, P = 0.02) in macrophages. Conjugated linoleic acid isomers can significantly reduce the activity of PLA(2) in macrophages and downregulate sPLA(2) expression. The consequence of this effect may be reduction of releasing the arachidonic acid (AA) from the cellular membranes of macrophages.
本研究的目的是评估共轭亚油酸异构体（CLA）对人巨噬细胞中磷脂酶A2（PLA2）表达和活性的影响。将巨噬细胞与30微摩尔顺式-9，反式-11和反式-10，顺式-12 CLA孵育48小时。孵育后，测量磷脂酶的总活性以及胞质型（cPLA2）和分泌型（sPLA2）磷脂酶的mRNA表达和sPLA2的活性。两种CLA异构体均降低了PLA2的总活性（顺式-9，反式-11 CLA降低30.2％，P <0.01；反式-10，顺式-12 CLA降低30％，P <0.001）。反式-10，顺式-12 CLA异构体下调了巨噬细胞中sPLA2的mRNA表达并降低了该酶的酶活性（降低23％，P = 0.02）。共轭亚油酸异构体可显著降低巨噬细胞中PLA2的活性并下调sPLA2的表达。这种作用的结果可能是减少巨噬细胞膜中花生四烯酸（AA）的释放。
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