从急性腹泻儿童中分离出的产肠毒素脆弱拟杆菌的bft基因分型
bft gene subtyping in enterotoxigenic Bacteroides fragilis isolated from children with acute diarrhea.
作者信息
Nakano Viviane, Gomes Tânia A T, Vieira Mônica A M, Ferreira Rita de Cássia, Avila-Campos Mario Julio
机构信息
Laboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
出版信息
Anaerobe. 2007 Feb;13(1):1-5. doi: 10.1016/j.anaerobe.2006.10.002. Epub 2006 Dec 12.
Enterotoxigenic Bacteroides fragilis (ETBF) strains are associated with diarrhea disease in farm animals and young children. In this study, the bft gene subtyping from ETBF strains recovered from one immunodeficient and two immunocompetent children with diarrhea were determined. Thirteen ETBF strains were isolated and by using a multiplex-PCR their bft subtypes were determined. All 13 ETBF strains harbored the bft-1 subtype and by AP-PCR they were clustered in the same group I. This study shows that ETBF strains can be present in acute diarrhea and that bft-1 subtype is often present in these organisms. However, further studies are needed to evaluate the role of this bft-1 subtype in the pathogenesis of diarrhea.
产肠毒素脆弱拟杆菌（ETBF）菌株与农场动物及幼儿的腹泻病有关。在本研究中，对从一名免疫缺陷和两名免疫功能正常的腹泻儿童中分离出的ETBF菌株进行了bft基因分型测定。分离出13株ETBF菌株，并通过多重聚合酶链反应确定了它们的bft亚型。所有13株ETBF菌株均携带bft-1亚型，且通过扩增片段长度多态性聚合酶链反应（AP-PCR），它们被聚类在同一组（I组）。本研究表明，ETBF菌株可存在于急性腹泻中，且bft-1亚型常在这些菌株中出现。然而，需要进一步研究来评估该bft-1亚型在腹泻发病机制中的作用。
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