Department of Thoracic Surgery, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark.
Wound Repair Regen. 1994 Jan;2(1):20-30. doi: 10.1046/j.1524-475X.1994.20105.x.
Subcutaneous insertion of expanded polytetrafluoroethylene tubes with local anesthesia constitutes a minimally invasive model permitting quantitative and qualitative studies of the wound healing potential in human beings. Light and electron microscopic examination of these implants in rats showed a normally appearing granulation tissue. The amount of hydroxyproline accumulated in the expanded polytetrafluoroethylene tubes may be assayed and converted to collagen content. In 28 surgical patients, the collagen content increased from 6.6 microg/cm (interquartile range: 5.1 to 9.9) after 5 days to 12.4 microg/cm (7.1 to 16.8) after 10 days (p < 0.05). The median ratio between the higher and lower collagen amount measured in two expanded polytetrafluoroethylene tubes inserted for an identical period within the same patient was 1.25 (1.10 to 1.81). This variability ratio was not related to the amount of collagen accumulated or the duration of implantation. There was a tendency for higher collagen amounts in the middle section than in the ends of the expanded polytetrafluoroethylene tubes, with a median ratio between the two sections equaling 1.12 (0.96 to 1.58); (p > 0.05). No infectious or hemorrhagic complications from the insertion occurred in any of the patients. Increments of collagen deposition with time may be easily assessed by this expanded polytetrafluoroethylene tube model, which is inexpensive and has high patient acceptance. Measurement variability was encountered, which has to be taken into account when designing clinical trials.
皮下插入局部麻醉膨胀聚四氟乙烯管构成了一种微创模型，允许对人类伤口愈合潜力进行定量和定性研究。这些植入物在大鼠中的光镜和电子显微镜检查显示出正常的肉芽组织。可测定并将膨胀聚四氟乙烯管中积累的羟脯氨酸量转换为胶原蛋白含量。在 28 例手术患者中，胶原含量从第 5 天的 6.6 微克/厘米（四分位数范围：5.1 至 9.9）增加到第 10 天的 12.4 微克/厘米（7.1 至 16.8）（p < 0.05）。同一患者同一时期内插入的两个膨胀聚四氟乙烯管中测量的较高和较低胶原量之间的中位数比值为 1.25（1.10 至 1.81）。该可变性比值与积累的胶原蛋白量或植入持续时间无关。在膨胀聚四氟乙烯管的中间部分比两端部分有更高的胶原量的趋势，两个部分之间的中位数比值等于 1.12（0.96 至 1.58）；（p > 0.05）。任何患者都没有因插入而发生感染或出血并发症。通过这种膨胀聚四氟乙烯管模型可以很容易地评估随时间增加的胶原蛋白沉积增量，该模型既便宜又具有高患者接受度。在设计临床试验时，必须考虑到遇到的测量可变性。
