He Jian-Qing, Wu Yun-Feng, Zhang Ge-Jie
College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China.
Wei Sheng Wu Xue Bao. 2006 Oct;46(5):773-7.
The soil samples were collected from primeval forest, alpine meadow, marshy, grainfield and vegetable farmland ect. 9 different kinds of Vegetative type and from 2970 - 4590 altitude of southeastern of Tibet. The amount,composition, antimicrobial activity of mesophilic and psychrophilic actinomycetes and some mesophilic actinomycetes of physiological and biochemical characteristics were studied by normal methods. These actinomycetes were identified at genus level by their morphological properties. Results indicated that: (1) 9 genus of actinomycetes were isolated from soil and Actinosynnema was never reported in China. Grain field contains the largest quantity of actinomycetes. (2) Primeval forest contains the largest quantity of antimicrobal actinomycetes, so more and more antimicrobal actinomycetes information can be obtained from them. (3) The strains tested had stronger antimicrobial activity against Gram-positive bacteria than Gram-negative bacteria, had stronger antimicrobial activity against fungi than bacteria. (4) Many strains of streptomyces have some enzyme activities such as gelatin utilization, Milk-clotting enzyme etc.
土壤样本采自西藏东南部海拔2970 - 4590米的原始森林、高山草甸、沼泽地、粮田和蔬菜农田等9种不同植被类型的区域。采用常规方法对嗜温放线菌和嗜冷放线菌的数量、组成、抗菌活性以及部分嗜温放线菌的生理生化特性进行了研究。通过形态学特征对这些放线菌进行了属水平的鉴定。结果表明:(1)从土壤中分离出9属放线菌,其中束丝放线菌在中国未见报道。粮田中的放线菌数量最多。(2)原始森林中抗菌放线菌数量最多,因此可以从其中获取越来越多的抗菌放线菌信息。(3)受试菌株对革兰氏阳性菌的抗菌活性强于革兰氏阴性菌,对真菌的抗菌活性强于细菌。(4)许多链霉菌菌株具有一些酶活性,如明胶利用、凝乳酶等。