乳糖阻遏物的“冷敏感”突变体
"Cold-sensitive" mutants of the Lac repressor.
作者信息
Barker Andrew, Oehler Stefan, Müller-Hill Benno
机构信息
Institut für Genetik der Universität zu Köln, Germany.
出版信息
J Bacteriol. 2007 Mar;189(5):2174-5. doi: 10.1128/JB.01462-06. Epub 2006 Dec 15.
Abstract
Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.
摘要
通过抑制琥珀色无义突变体产生的4000多个乳糖阻遏物单氨基酸替换突变体中，有13个被鉴定为具有冷敏感表型。然而，当作为错义突变表达时，这些替换均不会导致冷敏感性，这表明抑制机制是造成这种表型的原因。
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