SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning.

作者信息

Tachibana Akira, Tanabe Toshizumi, Yamauchi Kiyoshi

机构信息

Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.

出版信息

Anal Biochem. 2007 Feb 1;361(1):143-5. doi: 10.1016/j.ab.2006.10.044. Epub 2006 Nov 27.

DOI:10.1016/j.ab.2006.10.044

PMID:17173852

Abstract

摘要

相似文献

SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning.

Anal Biochem. 2007 Feb 1;361(1):143-5. doi: 10.1016/j.ab.2006.10.044. Epub 2006 Nov 27.

DNA methyltransferases as probes of chromatin structure in vivo.

Methods Enzymol. 1999;304:431-47. doi: 10.1016/s0076-6879(99)04026-4.

PCR method for generating multiple mutations at adjacent sites.

Methods Mol Biol. 2002;192:207-16. doi: 10.1385/1-59259-177-9:207.

DNA methylation analysis by bisulfite conversion, cloning, and sequencing of individual clones.

Methods Mol Biol. 2009;507:177-87. doi: 10.1007/978-1-59745-522-0_14.

Cloning PCR products. An overview.

Methods Mol Biol. 2002;192:111-9. doi: 10.1385/1-59259-177-9:111.

Rapid cDNA cloning by PCR screening (RC-PCR).

Methods Mol Biol. 2002;192:385-9. doi: 10.1385/1-59259-177-9:385.

Cloning of homologous genes by gene-capture PCR.

Methods Mol Biol. 2002;192:359-76. doi: 10.1385/1-59259-177-9:359.

A T-linker strategy for modification and directional cloning of PCR products.

Methods Mol Biol. 2003;226:475-84. doi: 10.1385/1-59259-384-4:475.

A high-throughput genome-walking method and its use for cloning unknown flanking sequences.

Anal Biochem. 2008 Oct 15;381(2):248-53. doi: 10.1016/j.ab.2008.07.012. Epub 2008 Jul 22.

In-frame cloning of synthetic genes using PCR inserts.

Methods Mol Biol. 2002;192:175-87. doi: 10.1385/1-59259-177-9:175.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

相似文献

SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning.

Anal Biochem. 2007 Feb 1;361(1):143-5. doi: 10.1016/j.ab.2006.10.044. Epub 2006 Nov 27.

DNA methyltransferases as probes of chromatin structure in vivo.

Methods Enzymol. 1999;304:431-47. doi: 10.1016/s0076-6879(99)04026-4.

PCR method for generating multiple mutations at adjacent sites.

Methods Mol Biol. 2002;192:207-16. doi: 10.1385/1-59259-177-9:207.

DNA methylation analysis by bisulfite conversion, cloning, and sequencing of individual clones.

Methods Mol Biol. 2009;507:177-87. doi: 10.1007/978-1-59745-522-0_14.

Cloning PCR products. An overview.

Methods Mol Biol. 2002;192:111-9. doi: 10.1385/1-59259-177-9:111.

Rapid cDNA cloning by PCR screening (RC-PCR).

Methods Mol Biol. 2002;192:385-9. doi: 10.1385/1-59259-177-9:385.

Cloning of homologous genes by gene-capture PCR.

Methods Mol Biol. 2002;192:359-76. doi: 10.1385/1-59259-177-9:359.

A T-linker strategy for modification and directional cloning of PCR products.

Methods Mol Biol. 2003;226:475-84. doi: 10.1385/1-59259-384-4:475.

A high-throughput genome-walking method and its use for cloning unknown flanking sequences.

Anal Biochem. 2008 Oct 15;381(2):248-53. doi: 10.1016/j.ab.2008.07.012. Epub 2008 Jul 22.

In-frame cloning of synthetic genes using PCR inserts.

Methods Mol Biol. 2002;192:175-87. doi: 10.1385/1-59259-177-9:175.