来自伊朗的一株超强毒传染性法氏囊病病毒分离株vp2基因的克隆与核苷酸分析

Cloning and nucleotide analysis of the vp2 gene of a very virulent infectious bursal disease virus isolate from Iran.

作者信息

Shamsara M, Ghorashi S A, Ahmadian G

机构信息

National Institute for Genetic Engineering and Biotechnology, Teheran, Iran.

出版信息

Acta Virol. 2006;50(4):229-34.

PMID:17177607

Abstract

An Iranian field isolate (IR01) of Infectious bursal disease virus (IBDV) was characterized by sequence analysis of its VP2 gene and protein. Comparison of the obtained sequences with those of IBDV isolates from other countries revealed that IR01 was similar to very virulent IBDV (vvIBDV) strains with the identities at nucleotide and amino acid levels reaching 98.198.9% and 99.199.3%, respectively. On the other hand, it was less similar to non-vvIBDV strains; with nucleotide and amino acid identities of 95.295.7% and 96.097.3%, respectively. Out of nine unique nucleotide differences found between IR01 and some other serotype 1 strains only two resulted in amino acid substitutions (Ile296Val and Thr359Lys). In phylogenetic analysis, IR01 was closely related to Asian and European vvIBDV strains. Based on these results, IR01 obviously belongs to vvIBDV strains.

摘要

通过对传染性法氏囊病病毒（IBDV）的一株伊朗田间分离株（IR01）的VP2基因和蛋白进行序列分析来对其进行特征鉴定。将获得的序列与来自其他国家的IBDV分离株的序列进行比较，结果显示IR01与超强毒IBDV（vvIBDV）毒株相似，其核苷酸和氨基酸水平的同一性分别达到98.1% - 98.9%和99.1% - 99.3%。另一方面，它与非vvIBDV毒株的相似性较低，核苷酸和氨基酸同一性分别为95.2% - 95.7%和96.0% - 97.3%。在IR01与其他一些血清型1毒株之间发现的9个独特核苷酸差异中，只有两个导致了氨基酸替换（Ile296Val和Thr359Lys）。在系统发育分析中，IR01与亚洲和欧洲的vvIBDV毒株密切相关。基于这些结果，IR01显然属于vvIBDV毒株。