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在肾脏发育过程中,Rho激酶在输尿管芽和后肾间充质形态发生的不同阶段发挥作用。

Rho kinase acts at separate steps in ureteric bud and metanephric mesenchyme morphogenesis during kidney development.

作者信息

Meyer Tobias N, Schwesinger Catherine, Sampogna Rosemary V, Vaughn Duke A, Stuart Robert O, Steer Dylan L, Bush Kevin T, Nigam Sanjay K

机构信息

Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0693, USA.

出版信息

Differentiation. 2006 Dec;74(9-10):638-47. doi: 10.1111/j.1432-0436.2006.00102.x.

Abstract

In this study, five different in vitro assays, which together recapitulate much of kidney development, were used to examine the role of the Rho-associated protein serine/threonine kinase (ROCK) in events central to ureteric bud (UB) and metanephric mesenchyme (MM) morphogenensis, in isolation and together. ROCK activity was found to be critical for (1) cell proliferation, growth, and development of the whole embryonic kidney in organ culture, (2) tip and stalk formation in cultures of isolated UBs, and (3) migration of MM cells (in a novel MM migration assay) during their condensation at UB tips (in a UB/MM recombination assay). Together, the data indicate selective involvement of Rho/ROCK in distinct morphogenetic processes necessary for kidney development and that the coordination of these events by Rho/ROCK provides a potential mechanism to regulate overall branching patterns, nephron formation, and thus, kidney architecture.

摘要

在本研究中,使用了五种不同的体外试验,这些试验共同概括了肾脏发育的大部分过程,以单独和共同研究Rho相关蛋白丝氨酸/苏氨酸激酶(ROCK)在输尿管芽(UB)和后肾间充质(MM)形态发生的核心事件中的作用。发现ROCK活性对于(1)器官培养中整个胚胎肾脏的细胞增殖、生长和发育,(2)分离的输尿管芽培养物中的尖端和柄部形成,以及(3)在输尿管芽/后肾间充质重组试验中,后肾间充质细胞在输尿管芽尖端凝聚期间的迁移(在一种新的后肾间充质迁移试验中)至关重要。总体而言,数据表明Rho/ROCK选择性参与肾脏发育所需的不同形态发生过程,并且Rho/ROCK对这些事件的协调提供了一种潜在机制来调节整体分支模式、肾单位形成,进而调节肾脏结构。

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