人鼻息肉组织中肌腱蛋白C的表达上调与嗜酸性粒细胞衍生的转化生长因子β1有关。
The up-regulated expression of tenascin C in human nasal polyp tissues is related to eosinophil-derived transforming growth factor beta1.
作者信息
Liu Zheng, Lu Xiang, Wang Heng, Gao Qixue, Cui Yonghua
机构信息
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
出版信息
Am J Rhinol. 2006 Nov-Dec;20(6):629-33. doi: 10.2500/ajr.2006.20.2918.
BACKGROUND
Tissue remodeling is an important characteristic of nasal polyps (NPs). However, the mechanisms underlying the remodeling processes are poorly defined. This study investigated the role of transforming growth factor (TGF) beta1 and eosinophils in the expression of tenascin C (Tn-C), an extracellular matrix glycoprotein, in NPs.
METHODS
The protein expression of Tn-C and TGF-beta1 was examined by means of immunohistochemistry in NPs and normal control inferior turbinate tissues. Furthermore, cell culture, quantitative RT-PCR, and in situ immunocytofluorescence techniques were used to investigate the direct effect of TGF-beta1 and eosinophils on Tn-C production in primary nasal epithelial cells.
RESULTS
Tn-C protein expression was significantly up-regulated in NP tissues and correlated with TGF-beta1+ eosinophils. TGF-beta1 and eosinophils dramatically induced Tn-C mRNA and protein expression in nasal epithelial cells. The effect of eosinophils could be inhibited partly by a neutralizing antibody to TGF-beta1.
CONCLUSION
Eosinophil-derived TGF-beta1 may contribute, at least in part, to the tissue remodeling in NPs.
背景
组织重塑是鼻息肉（NP）的一个重要特征。然而，重塑过程背后的机制尚不清楚。本研究调查了转化生长因子（TGF）β1和嗜酸性粒细胞在NP中细胞外基质糖蛋白腱生蛋白C（Tn-C）表达中的作用。
方法
通过免疫组织化学检测NP和正常对照下鼻甲组织中Tn-C和TGF-β1的蛋白表达。此外，采用细胞培养、定量逆转录聚合酶链反应（RT-PCR）和原位免疫细胞荧光技术研究TGF-β1和嗜酸性粒细胞对原代鼻上皮细胞中Tn-C产生的直接影响。
结果
NP组织中Tn-C蛋白表达显著上调，且与TGF-β1+嗜酸性粒细胞相关。TGF-β1和嗜酸性粒细胞显著诱导鼻上皮细胞中Tn-C mRNA和蛋白表达。嗜酸性粒细胞的作用可被TGF-β1中和抗体部分抑制。
结论
嗜酸性粒细胞衍生的TGF-β1可能至少部分促成NP中的组织重塑。
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