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网脉臂形草(图&德诺特)斯塔夫(禾本科:黍亚科)的小孢子发生

Microsporogenesis in Brachiaria dictyoneura (Fig. & De Not.) Stapf (Poaceae: Paniceae).

作者信息

Risso-Pascotto C, Pagliarini M S, Valle C B

机构信息

Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Maringá, PR, Brasil.

出版信息

Genet Mol Res. 2006 Dec 12;5(4):837-45.

Abstract

Microsporogenesis was analyzed in five accessions of Brachiaria dictyoneura presenting x = 6 as the basic chromosome number. All accessions were tetraploid (2n = 4x = 24) with chromosome pairing in bi-, tri-, and quadrivalents. The recorded meiotic abnormalities were those typical of polyploids, including precocious chromosome migration to the poles, laggard chromosomes, and micronucleus formation. The frequency of these abnormalities, however, was lower than those reported for other polyploid accessions previously analyzed for other Brachiaria species. Cell fusion and absence of cytokinesis were also recorded in some accessions, leading to restitutional nucleus formation in some cells. Genetically unbalanced microspores, binucleate, and 2n microspores were found among normal meiotic products as results from these abnormalities. The limitation in using these accessions as pollen donor in interspecific crosses with sexual species with x = 7 or x = 9 in breeding programs is discussed.

摘要

对五个二倍体臂形草(Brachiaria dictyoneura)材料进行了小孢子发生分析,其基本染色体数x = 6。所有材料均为四倍体(2n = 4x = 24),染色体配对出现二价体、三价体和四价体。记录到的减数分裂异常是多倍体典型的异常,包括染色体早熟向两极迁移、落后染色体和微核形成。然而,这些异常的频率低于之前对其他臂形草物种分析的其他多倍体材料所报道的频率。在一些材料中还记录到细胞融合和胞质分裂缺失,导致一些细胞中形成 restitutional 核。由于这些异常,在正常减数分裂产物中发现了遗传不平衡的小孢子、双核小孢子和2n小孢子。讨论了在育种计划中,将这些材料用作与x = 7或x = 9的有性物种进行种间杂交的花粉供体的局限性。

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