Sugihara Izumi, Quy Pham Nguyen
Department of Systems Neurophysiology, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, Japan.
J Comp Neurol. 2007 Feb 20;500(6):1076-92. doi: 10.1002/cne.21219.
Aldolase C (zebrin II) is expressed in Purkinje cells aligned in complicated longitudinal stripe-shaped compartments. The tight link between these aldolase C compartments and the topographic olivocerebellar projection to them has made it possible to identify each compartment as a target of a specific subarea of the inferior olive and thus as a functionally distinct entity in the rat. However, it is unknown whether the overall organization of aldolase C compartments is preserved in other mammals. In this study, we tried to clarify this organization in the mouse, which is more useful in genetic studies than the rat, by identifying each aldolase C compartment in terms of the olivocerebellar projection pattern. First, aldolase C compartments were reconstructed from serial sections throughout the cerebellar cortex. Aldolase C and olivocerebellar climbing fibers were then doubly labeled by small injections of biotinylated dextran amine into various areas of the inferior olive. Climbing fibers were topographically distributed on a specific linked pair of aldolase C compartments in the rostral and caudal cerebellum. The overall relationship between aldolase C compartments and the topographic olivocerebellar projection to them in the mouse was similar to that in the rat, except for some minor differences, suggesting that the aldolase C compartments and olivocerebellar projection are organized according to a common fundamental organization in the mouse and rat. This allowed the unequivocal identification of all aldolase C compartments in the mouse by referring to the definition and nomenclature in the rat.
醛缩酶C（zebrin II）在排列成复杂纵向条纹状区室的浦肯野细胞中表达。这些醛缩酶C区室与向它们的地形学橄榄小脑投射之间的紧密联系，使得在大鼠中能够将每个区室识别为下橄榄特定亚区的靶标，从而将其识别为功能上不同的实体。然而，在其他哺乳动物中醛缩酶C区室的整体组织是否得以保留尚不清楚。在本研究中，我们试图通过根据橄榄小脑投射模式识别小鼠中的每个醛缩酶C区室，来阐明这种组织，小鼠在基因研究中比大鼠更有用。首先，从整个小脑皮质的连续切片重建醛缩酶C区室。然后通过将生物素化葡聚糖胺小剂量注射到下橄榄的各个区域，对醛缩酶C和橄榄小脑攀缘纤维进行双重标记。攀缘纤维在小脑头端和尾端特定相连的一对醛缩酶C区室上呈地形学分布。除了一些细微差异外，小鼠中醛缩酶C区室与向它们的地形学橄榄小脑投射之间的整体关系与大鼠相似，这表明醛缩酶C区室和橄榄小脑投射在小鼠和大鼠中是根据共同的基本组织方式进行组织的。这使得通过参考大鼠中的定义和命名法能够明确识别小鼠中的所有醛缩酶C区室。
