Koszinowski U, Volkmann B, Thomssen R
Z Immunitatsforsch Exp Klin Immunol. 1975 May;148(5):451-61.
Cell mediated immunity to vaccinia virus in man was studied by lymphocyte transformation. Vaccinia antigen, propagated on BHK-21 and Vero cells, could be used successfully for in-vitro testing after partial purification as well as crude infectious homogenates. Vaccinia antigen preparations were effective both in the infective and the inactivated state. Inactivation was usually accompanied with a certain loss of stimulating activity. Development of cell mediated immune response in-vitro after first vaccination was investigated in 17 adults. Vaccinia virus specific lymphocyte transformation was seen in the second week after vaccination in all cases. Following revaccination no increase of lymphocyte transformation ratio could be observed in 11 persons studied. At the same time the titers of humoral antibodies were elevated.
通过淋巴细胞转化研究了人对痘苗病毒的细胞介导免疫。在BHK - 21和Vero细胞上繁殖的痘苗抗原，经部分纯化以及粗制感染性匀浆后，可成功用于体外检测。痘苗抗原制剂在感染状态和灭活状态下均有效。灭活通常伴随着一定程度的刺激活性丧失。对17名成年人首次接种疫苗后体外细胞介导免疫反应的发展进行了研究。所有病例在接种疫苗后第二周均出现痘苗病毒特异性淋巴细胞转化。在研究的11人中，再次接种后未观察到淋巴细胞转化率增加。与此同时，体液抗体滴度升高。
