利用单克隆抗体建立尿胰蛋白酶抑制剂的酶联免疫吸附测定法。
Establishment of an enzyme-linked immuno-sorbent assay for urinary trypsin inhibitor by using a monoclonal antibody.
作者信息
Trefz G, Streit B, Justus C W, Ebert W, Kramer M D
机构信息
Thoraxklinik Heidelberg-Rohrbach, Heidelberg-Rohrbach.
出版信息
J Immunoassay. 1991;12(3):347-69. doi: 10.1080/01971529108055077.
Monoclonal antibodies against inter-alpha-trypsin-inhibitor (ITI) were produced. One clone showing specificity for urinary trypsin inhibitor (UTI), a proteolytic fragment of ITI, which is excreted into urine, was selected for the establishment of an enzyme-linked immuno-sorbent assay (ELISA). The ELISA for the quantification of UTI was shown to work reproducibly in the range between 0.5 and 10 ng UTI/ml urine. Urines of several patients suffering from different lung diseases were screened for UTI using the established ELISA. Highest UTI levels were found in the urine of patients with lung empyema. A more moderate increase was observed in patients suffering from lung tuberculosis and from secondary and primary lung tumors.
制备了抗α-胰蛋白酶抑制剂（ITI）的单克隆抗体。选择了一个对尿胰蛋白酶抑制剂（UTI）具有特异性的克隆，UTI是ITI的一种蛋白水解片段，可排泄到尿液中，用于建立酶联免疫吸附测定（ELISA）。用于定量UTI的ELISA在0.5至10 ng UTI/ml尿液范围内具有可重复性。使用已建立的ELISA对几名患有不同肺部疾病的患者的尿液进行UTI筛查。在肺脓肿患者的尿液中发现UTI水平最高。在患有肺结核以及继发性和原发性肺部肿瘤的患者中观察到UTI水平有较为适度的升高。
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