Jakubík Jan, Dolezal Vladimír
Department of Neurochemistry, Institute of Physiology, Academy of Sciences of the Czech Republic, CZ-14220 Prague, Czech Republic.
J Mol Neurosci. 2006;30(1-2):111-2. doi: 10.1385/JMN:30:1:111.
It has been shown previously that the third extracellular loop (o3) and its vicinity play a critical role in allosteric modulation at muscarinic acetylcholine receptors (mAChRs) (Ellis et al., 1993; Krejçí and Tuçek, 2001; Buller et al., 2002). In this study interaction of four chemically related substances (strychnine, its dimethoxy derivate brucine, precursor for synthesis of strychnine Wieland-Gumlich aldehyde (WGA), and precursor for synthesis of alcuronium propargyl-WGA) with orthosteric antagonist N-methylscopolamine (NMS) was investigated on the M3 subtype of mAChRs mutated at the o3 loop.