Human SLUG does not directly bind to CtBP1.
作者信息
Bailey Charvann K, Misra Smita, Mittal Mukul K, Chaudhuri Gautam
机构信息
Division of Cancer Biology, Department of Biomedical Sciences, Meharry Medical College, Nashville, TN 37208, USA.
出版信息
Biochem Biophys Res Commun. 2007 Feb 16;353(3):661-4. doi: 10.1016/j.bbrc.2006.12.097. Epub 2006 Dec 22.
Abstract
SLUG is a transcriptional repressor protein implicated to have major role in the oncogenesis and metastasis of human breast cells. We previously have shown by chromatin immunoprecipitation assay that human SLUG (hSLUG) is co-localized with the co-repressor protein CtBP1 as bound to the BRCA2 gene silencer [M.K. Tripathi, S. Misra, S.V. Khedkar, N. Hamilton, C. Irvin-Wilson,, C. Sharan, L. Sealy, G. Chaudhuri, J. Biol. Chem. 280 (2005) 17163-17171]. hSLUG was predicted to be binding directly to CtBP1 because of an apparent presence of CtBP1 binding site in its amino acid sequences. Here, we provide evidence through yeast two-hybrid and in vitro co-immunoprecipitation analyses that hSLUG does not directly interacts with hCtBP1. This observation will help in the study of the mode of action of hSLUG in human cells.
摘要
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Cell. 2005 Nov 18;123(4):641-53. doi: 10.1016/j.cell.2005.09.029.
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Biochem J. 2006 Mar 1;394(Pt 2):449-57. doi: 10.1042/BJ20050591.
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Development. 2005 Jul;132(14):3151-61. doi: 10.1242/dev.01907.
5
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J Steroid Biochem Mol Biol. 2005 May;95(1-5):17-23. doi: 10.1016/j.jsbmb.2005.04.002.
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