Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library.

作者信息

Dezorzová-Tomanová K, Molinková D, Pekarová M, Celer V, Smola J

机构信息

Institute of Microbiology and Immunology, Faculty of Veterinary Medicine, Veterinary and Pharmaceutical University Brno, 612 42 Brno, Czech Republic.

出版信息

Res Vet Sci. 2007 Aug;83(1):85-90. doi: 10.1016/j.rvsc.2006.11.002. Epub 2007 Jan 2.

DOI:10.1016/j.rvsc.2006.11.002

PMID:17198717

Abstract

Single-chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against L. intracellularis.

摘要