Ohtani A, Arai Y, Ozoe F, Ohta H, Narusuye K, Huang J, Enomoto K, Kataoka H, Hirota A, Ozoe Y
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan.
Insect Mol Biol. 2006 Dec;15(6):763-72. doi: 10.1111/j.1365-2583.2006.00676.x.
A cDNA encoding an octopamine (OA) receptor (BmOAR1) was isolated from the nerve tissue of silkworm (Bombyx mori) larvae. Comparison of amino acid sequences showed that BmOAR1 is highly identical to OA receptors isolated from Periplaneta americana (Pa oa(1)), Apis mellifera (AmOA1), and Drosophila melanogaster (OAMB or DmOA1A). BmOAR1 was stably expressed in HEK-293 cells. OA above 1 microM led to an increase in intracellular cyclic AMP concentration (cAMP). The synthetic OA-receptor agonist demethylchlordimeform also elevated cAMP to the same maximal level (approximately 5-fold over the basal level) as that induced by OA. However, other biogenic amines, tyramine and dopamine, and chlordimeform were without effects. The cAMP level raised by OA was lowered by antagonists; the rank order of antagonist activity was chlorpromazine > mianserin = yohimbine. Cyproheptadine and metoclopramide had little effect. OA above 100 nM induced a transient or sustained increase in intracellular Ca(2+) concentration (Ca(2+)), depending on the concentration of OA. Sequence homology and functional analysis data indicate that BmOAR1 is an alpha-adrenergic-like OA receptor of B. mori.
从家蚕（Bombyx mori）幼虫的神经组织中分离出一种编码章鱼胺（OA）受体（BmOAR1）的cDNA。氨基酸序列比较表明，BmOAR1与从美洲大蠊（Periplaneta americana，Pa oa(1)）、意大利蜜蜂（Apis mellifera，AmOA1）和黑腹果蝇（Drosophila melanogaster，OAMB或DmOA1A）中分离出的OA受体高度同源。BmOAR1在HEK-293细胞中稳定表达。1 microM以上的OA导致细胞内环状AMP浓度（cAMP）升高。合成的OA受体激动剂去甲基杀虫脒也将cAMP升高到与OA诱导的相同最大水平（比基础水平高约5倍）。然而，其他生物胺、酪胺和多巴胺以及杀虫脒没有作用。OA升高的cAMP水平被拮抗剂降低；拮抗剂活性的顺序为氯丙嗪>米安色林 = 育亨宾。赛庚啶和甲氧氯普胺作用很小。100 nM以上的OA根据OA的浓度诱导细胞内Ca(2+)浓度（Ca(2+)）短暂或持续升高。序列同源性和功能分析数据表明，BmOAR1是家蚕的一种α-肾上腺素能样OA受体。
