Guo Yurong, Fu Zongming, Van Eyk Jennifer E
Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21224, USA.
Proc Am Thorac Soc. 2007 Jan;4(1):9-17. doi: 10.1513/pats.200608-156JG.
Proteomics is a rapidly developing field and it opens new horizons in many research areas of life sciences. In the field of medicine, proteomics promises to accelerate the discovery of new drug targets and protein disease markers useful for in vitro diagnosis. In this article, we review the current proteomics technologies for biomarker discovery and validation, which include two-dimensional gel electrophoresis, one- and two-dimensional liquid chromatography, and proteomic microarrays. We will also review proteomic strategies for protein-protein interactions and identification of post-translational modifications. Selection of the more effective technology or combination of technologies is required to maximize the interpretation and utility of the data.
蛋白质组学是一个快速发展的领域,它在生命科学的许多研究领域开辟了新的视野。在医学领域,蛋白质组学有望加速新药物靶点的发现以及有助于体外诊断的蛋白质疾病标志物的发现。在本文中,我们综述了用于生物标志物发现和验证的当前蛋白质组学技术,包括二维凝胶电泳、一维和二维液相色谱以及蛋白质组芯片。我们还将综述蛋白质-蛋白质相互作用以及翻译后修饰鉴定的蛋白质组学策略。需要选择更有效的技术或技术组合,以最大限度地解读数据并发挥其效用。