Babujee Lavanya, Venkatesh Balakrishnan, Yamazaki Akihiro, Tsuyumu Shinji
Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
J Proteome Res. 2007 Jan;6(1):62-9. doi: 10.1021/pr060423l.
We present results of the first comprehensive proteomic analysis of the outer membrane of the bacterial phytopathogen Dickeya dadantii strain 3937 and its response to virulence-contributing factors such as host plant extract, acidic stress, and iron starvation. We analyzed the carbonate-insoluble membrane fractions, which are highly enriched for outer membrane proteins, using two-dimensional electrophoresis and identified the proteins by MALDI-TOF MS. Forty unique proteins were identified, some of which were differentially expressed under the above conditions.
我们展示了对植物病原细菌达旦氏果胶杆菌3937菌株外膜进行首次全面蛋白质组分析的结果，以及该菌株对宿主植物提取物、酸性胁迫和铁饥饿等毒力相关因子的反应。我们使用二维电泳分析了富含外膜蛋白的碳酸盐不溶性膜组分，并通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定了这些蛋白质。共鉴定出40种独特的蛋白质，其中一些在上述条件下差异表达。
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