Rahman Raja Noor Zaliha Abdul, Baharum Syarul Nataqain, Salleh Abu Bakar, Basri Mahiran
Enzyme and Microbial Technology Research, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
J Microbiol. 2006 Dec;44(6):583-90.
In this study, an organic solvent tolerant bacterial strain was isolated. This strain was identified as Pseudomonas sp. strain S5, and was shown to degrade BTEX (Benzene, Toluene, Ethyl-Benzene, and Xylene). Strain S5 generates an organic solvent-tolerant lipase in the late logarithmic phase of growth. Maximum lipase production was exhibited when peptone was utilized as the sole nitrogen source. Addition of any of the selected carbon sources to the medium resulted in a significant reduction of enzyme production. Lower lipase generation was noted when an inorganic nitrogen source was used as the sole nitrogen source. This bacterium hydrolyzed all tested triglycerides and the highest levels of production were observed when olive oil was used as a natural triglyceride. Basal medium containing Tween 60 enhanced lipase production to the most significant degree. The absence of magnesium ions (Mg2+) in the basal medium was also shown to stimulate lipase production. Meanwhile, an alkaline earth metal ion, Na+, was found to stimulate the production of S5 lipase.
在本研究中，分离出了一株耐有机溶剂的细菌菌株。该菌株被鉴定为假单胞菌属菌株S5，并显示出能降解BTEX（苯、甲苯、乙苯和二甲苯）。菌株S5在生长的对数后期产生一种耐有机溶剂的脂肪酶。当蛋白胨用作唯一氮源时，脂肪酶产量最高。向培养基中添加任何一种选定的碳源都会导致酶产量显著降低。当使用无机氮源作为唯一氮源时，脂肪酶生成量较低。该细菌能水解所有测试的甘油三酯，当使用橄榄油作为天然甘油三酯时，观察到最高产量。含有吐温60的基础培养基能最大程度地提高脂肪酶产量。基础培养基中镁离子（Mg2+）的缺失也显示出能刺激脂肪酶的产生。同时，发现一种碱土金属离子Na+能刺激S5脂肪酶的产生。
