Cai Fangping, Chen Haifeng, Hicks Charles B, Bartlett John A, Zhu Jun, Gao Feng
Duke Human Vaccine Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.
Nat Methods. 2007 Feb;4(2):123-5. doi: 10.1038/nmeth995. Epub 2007 Jan 7.
We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.
我们开发了一种高灵敏度的平行等位基因特异性测序（PASS）检测方法，以同时分析大量病毒基因组，并检测约0.1-0.01%水平的少量耐药群体。在感染人类免疫缺陷病毒（HIV）个体的样本上使用该检测方法，我们成功检测并定量了耐药病毒的少量群体，并对多药耐药突变进行了连锁分析。该检测方法可作为研究HIV和其他感染因子耐药性的有用工具。
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