Norymberski J K, Stoddart N, Wolstenholme J B
Biochem J. 1975 Jan;145(1):47-51. doi: 10.1042/bj1450047.
A method of analysis is proposed based on the enzyme-catalysed transfer of tritium from [3H]NADH to suitable substrates. Its practicability is demonstrated on the examples of oestrone and progesterone with the respective use of the 3 beta, 17 beta- and 3 alpha, 20 beta-hydroxysteroid oxidoreductase. Specificity is tested by application to the analysis of the plasma of pregnant women and measurement of the 3H/14C ratios on purification of the enzymic reduction products.
提出了一种基于酶催化将氚从[3H]NADH转移至合适底物的分析方法。以雌酮和孕酮为例,分别使用3β,17β-和3α,20β-羟基类固醇氧化还原酶证明了其可行性。通过应用于孕妇血浆分析以及对酶促还原产物纯化后测量3H/14C比值来测试特异性。