通过用氚标记的还原型烟酰胺腺嘌呤二核苷酸对氧代甾体进行还原的放射酶法测定。
Radio-enzymic assay of oxo steroids by their reduction with the tritiated form of reduced nicotinamide--adenine dinucleotide.
作者信息
Norymberski J K, Stoddart N, Wolstenholme J B
出版信息
Biochem J. 1975 Jan;145(1):47-51. doi: 10.1042/bj1450047.
Abstract
A method of analysis is proposed based on the enzyme-catalysed transfer of tritium from [3H]NADH to suitable substrates. Its practicability is demonstrated on the examples of oestrone and progesterone with the respective use of the 3 beta, 17 beta- and 3 alpha, 20 beta-hydroxysteroid oxidoreductase. Specificity is tested by application to the analysis of the plasma of pregnant women and measurement of the 3H/14C ratios on purification of the enzymic reduction products.
摘要
提出了一种基于酶催化将氚从[3H]NADH转移至合适底物的分析方法。以雌酮和孕酮为例，分别使用3β,17β-和3α,20β-羟基类固醇氧化还原酶证明了其可行性。通过应用于孕妇血浆分析以及对酶促还原产物纯化后测量3H/14C比值来测试特异性。
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Radio-enzymic assay of oxo steroids by their reduction with the tritiated form of reduced nicotinamide--adenine dinucleotide.通过用氚标记的还原型烟酰胺腺嘌呤二核苷酸对氧代甾体进行还原的放射酶法测定。
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