• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过用氚标记的还原型烟酰胺腺嘌呤二核苷酸对氧代甾体进行还原的放射酶法测定。

Radio-enzymic assay of oxo steroids by their reduction with the tritiated form of reduced nicotinamide--adenine dinucleotide.

作者信息

Norymberski J K, Stoddart N, Wolstenholme J B

出版信息

Biochem J. 1975 Jan;145(1):47-51. doi: 10.1042/bj1450047.

DOI:10.1042/bj1450047
PMID:172065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165185/
Abstract

A method of analysis is proposed based on the enzyme-catalysed transfer of tritium from [3H]NADH to suitable substrates. Its practicability is demonstrated on the examples of oestrone and progesterone with the respective use of the 3 beta, 17 beta- and 3 alpha, 20 beta-hydroxysteroid oxidoreductase. Specificity is tested by application to the analysis of the plasma of pregnant women and measurement of the 3H/14C ratios on purification of the enzymic reduction products.

摘要

提出了一种基于酶催化将氚从[3H]NADH转移至合适底物的分析方法。以雌酮和孕酮为例，分别使用3β,17β-和3α,20β-羟基类固醇氧化还原酶证明了其可行性。通过应用于孕妇血浆分析以及对酶促还原产物纯化后测量3H/14C比值来测试特异性。

相似文献

1
Radio-enzymic assay of oxo steroids by their reduction with the tritiated form of reduced nicotinamide--adenine dinucleotide.通过用氚标记的还原型烟酰胺腺嘌呤二核苷酸对氧代甾体进行还原的放射酶法测定。
Biochem J. 1975 Jan;145(1):47-51. doi: 10.1042/bj1450047.
2
The steric course with respect to the reduced nicotinamide-adenine dinucleotide of the reduction of 3-oxo steroids catalysed by cortisone reductase.可的松还原酶催化的3-氧代甾体还原反应相对于还原型烟酰胺腺嘌呤二核苷酸的立体化学过程。
Eur J Biochem. 1973 Apr;34(2):395-400. doi: 10.1111/j.1432-1033.1973.tb02771.x.
3
Pyridine nucleotide levels under conditions of 5 alpha-dihydrotestosterone-stimulated 17 beta-estradiol formation from estrone and pathway of nicotinamide adenine dinucleotide biosynthesis in placental villi in vitro.体外胎盘绒毛中，在5α-二氢睾酮刺激雌酮生成17β-雌二醇的条件下的吡啶核苷酸水平及烟酰胺腺嘌呤二核苷酸生物合成途径。
J Clin Endocrinol Metab. 1991 Jul;73(1):140-5. doi: 10.1210/jcem-73-1-140.
4
[Specificity of 3 alpha, 20 beta-hydroxysteroid: nad+ -oxidoreductase (author's transl)].[3α,20β-羟基类固醇：NAD⁺氧化还原酶的特异性（作者译）]
J Clin Chem Clin Biochem. 1976 Feb;14(2):59-64.
5
20-ALPHA-HYDROXYSTEROID DEHYDROGENASE ACTIVITY A FUNCTION OF HUMAN PLACENTAL 17-BETA-HYDROXYSTEROID DEHYDROGENASE.
Biochim Biophys Acta. 1964 Sep 18;89:557-60.
6
A one-step enzymatic assay for the measurement of 17 beta-hydroxy- and 17-oxo-steroid profiles in biological samples.一种用于测量生物样品中17β-羟基和17-氧代甾体谱的一步酶促测定法。
J Steroid Biochem. 1986 Sep;25(3):403-10. doi: 10.1016/0022-4731(86)90253-0.
7
The stereospecificity of the enzymic reduction of 21-dehydrocortisol by NADH.
J Biol Chem. 1975 Oct 10;250(19):7547-53.
8
Enzymatic estimation of steroids in subpicomole quantities by hydroxysteroid dehydrogenases and nicotinamide nucleotide cycling.通过羟基类固醇脱氢酶和烟酰胺核苷酸循环对亚皮摩尔量的类固醇进行酶法测定。
J Biol Chem. 1982 Jan 25;257(2):633-42.
9
Analysis of free estrone and 17-beta-estradiol in plasma by tritiated sodium borohydride reduction.用氚化硼氢化钠还原法分析血浆中的游离雌酮和17-β-雌二醇。
Am J Obstet Gynecol. 1970 Feb 1;106(3):447-54. doi: 10.1016/0002-9378(70)90374-1.
10
A major influence of a 1 alpha, 2 alpha-methylene group on reduction of a steroid 3-oxo group by 2 nicotinamide-adenine dinucleotide-linked 3-hydroxy steroid oxidoreductases.1α,2α-亚甲基基团对两种烟酰胺腺嘌呤二核苷酸连接的3-羟基类固醇氧化还原酶还原类固醇3-氧代基团的主要影响。
Biochem J. 1972 Nov;130(1):37P-38P.

本文引用的文献

1
Stereospecificity of hydrogen transfer by pyridine nucleotide-linked hydroxysteroid dehydrogenases.
J Biol Chem. 1960 Jul;235:2147-51.
2
[20 beta-Hydroxysteroid dehydrogenase. 2. Preparation and crystallization].[20β-羟基类固醇脱氢酶。2. 制备与结晶]
Biochem Z. 1960;333:95-105.
3
[20 beta-Hydroxysteroid dehydrogenase, a new crystalline enzyme].[20β-羟基类固醇脱氢酶，一种新的结晶酶]
Biochim Biophys Acta. 1959 Sep;35:270-2. doi: 10.1016/0006-3002(59)90366-x.
4
The enzymatic transfer of hydrogen. IV. The reaction catalyzed by a beta-hydroxysteroid dehydrogenase.氢的酶促转移。IV. β-羟基类固醇脱氢酶催化的反应。
J Biol Chem. 1955 Feb;212(2):801-9.
5
The enzymatic transfer of hydrogen. III. The reaction catalyzed by malic dehydrogenase.氢的酶促转移。III. 苹果酸脱氢酶催化的反应。
J Biol Chem. 1955 Feb;212(2):787-800.
6
PURIFICATION OF STEROID-INDUCED ENZYMES FROM PSEUDOMONAS TESTOSTERONI.从睾丸酮假单胞菌中纯化甾体诱导酶
Biochim Biophys Acta. 1964 Sep 18;89:398-408. doi: 10.1016/0926-6569(64)90066-5.
7
STUDIES WITH THE HYDROXYSTEROID DEHYDROGENASES. I. A SIMPLIFIED METHOD FOR THE ENZYMATIC ESTIMATION OF 3- AND 17-HYDROXYSTEROIDS.羟基类固醇脱氢酶的研究。I. 一种用于酶法测定3-和17-羟基类固醇的简化方法。
J Clin Endocrinol Metab. 1964 Apr;24:367-74. doi: 10.1210/jcem-24-4-367.
8
[Use of Huebener's 20 beta-hydroxysteroid dehydrogenase in the microchemical identification and separation of steroids].[休布纳20β-羟基类固醇脱氢酶在类固醇的微量化学鉴定与分离中的应用]
Hoppe Seylers Z Physiol Chem. 1962 Dec 15;330:31-7. doi: 10.1515/bchm2.1962.330.1.31.
9
[The enzymic 20-keto-reduction by extracts from Streptomyces hydrogenans].
Hoppe Seylers Z Physiol Chem. 1958;313:124-9. doi: 10.1515/bchm2.1958.313.1.124.
10
Principles of the enzymatic measurement of steroids.甾体激素的酶促测定原理。
J Biol Chem. 1957 Jul;227(1):37-52.