Qiao You-jie, Zhu Jun-mei, Zhang Yu, Yuan Hong-yan
Tianhe Hospital, Tianjin 300050, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Jan;19(1):41-3.
To reproduce a rat model of disseminated intravascular coagulation (DIC) accompanied by multiple organ dysfunction syndrome (MODS) induced by endotoxin.
Twenty-four healthy Wistar rats were randomly divided into four groups: control group, low dosage lipopolysaccharide (LPS) group, middle dosage LPS group, and high dosage LPS group (each n=6). Rats of each group were given different dosages of normal saline (1.4 ml/kg, 2.8 ml/kg), low dosages LPS [1.4 mg/kg (56 microg/kg), 2.8 ml/kg (112 microg/kg)], middle dosages LPS [1.4 mg/kg(98 microg/kg), 2.8 ml/kg (196 microg/kg)] and high dosages LPS [1.4 ml/kg (196 microg/kg), 2.8 ml/kg (392 microg/kg)] respectively twice 12 hours apart through femoral vein intubation injection. Blood platelet (PLT) count, coagulation function, D-dimer, fibrinogen (Fbg), antithrombin III (AT-III) blood glucose (Glu), biochemistry indexes including aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were determined before and after LPS challenge, and histopathologic changes of lung, liver and kidney were observed 4 hours after the second injection.
The rats in high dosage LPS group died 4 hours later, and the rats in low and middle dosage LPS groups survived after double LPS challenge. The results of blood PLT, D-dimer, Fbg, Glu, AST, ALT, ALP, LDH, coagulation function of activated partial thromboplastin time, prothrombin time and level of anti-thrombin III in middle dosage LPS group were significantly different compared with those of control group (P<0.05 or P<0.01). Obvious changes in histopathology were found in major organs such as lung, kidney and liver.
Double intravenous LPS challenge (98 microg/kg and 196 microg/kg) in rats can reproduce a rat model with DIC accompanied by multiple organ injuries.
复制内毒素诱导的伴有多器官功能障碍综合征（MODS）的弥散性血管内凝血（DIC）大鼠模型。
将24只健康Wistar大鼠随机分为四组：对照组、低剂量脂多糖（LPS）组、中剂量LPS组和高剂量LPS组（每组n = 6）。通过股静脉插管注射，分别给每组大鼠不同剂量的生理盐水（1.4 ml/kg、2.8 ml/kg）、低剂量LPS [1.4 mg/kg（56 μg/kg）、2.8 ml/kg（112 μg/kg）]、中剂量LPS [1.4 mg/kg（98 μg/kg）、2.8 ml/kg（196 μg/kg）]和高剂量LPS [1.4 ml/kg（196 μg/kg）、2.8 ml/kg（392 μg/kg）]，间隔12小时注射两次。在LPS攻击前后测定血小板（PLT）计数、凝血功能、D-二聚体、纤维蛋白原（Fbg）、抗凝血酶III（AT-III）、血糖（Glu）以及包括转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和乳酸脱氢酶（LDH）在内的生化指标，并在第二次注射后4小时观察肺、肝和肾的组织病理学变化。
高剂量LPS组大鼠在4小时后死亡，低、中剂量LPS组大鼠在两次LPS攻击后存活。中剂量LPS组的血液PLT、D-二聚体、Fbg、Glu、AST、ALT、ALP、LDH、活化部分凝血活酶时间、凝血酶原时间的凝血功能以及抗凝血酶III水平与对照组相比有显著差异（P < 0.05或P < 0.01）。在肺、肾和肝等主要器官中发现了明显的组织病理学变化。
大鼠两次静脉注射LPS（98 μg/kg和196 μg/kg）可复制伴有多器官损伤的DIC大鼠模型。
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