基于固定在金膜电极上的二甲基亚砜还原酶构建二甲基亚砜传感器。

Construction of a dimethyl sulfoxide sensor based on dimethyl sulfoxide reductase immobilized on a au film electrode.

作者信息

Yonehara Hiroki, Fujii Shin-Ichiro, Sato Kiichi, Abo Mitsuru, Yoshimura Etsuro

机构信息

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

出版信息

Anal Sci. 2007 Jan;23(1):55-8. doi: 10.2116/analsci.23.55.

DOI:10.2116/analsci.23.55

PMID:17213624

Abstract

A novel dimethyl sulfoxide (DMSO) sensor using DMSO reductase and film electrodes was constructed. The Au and Ag electrodes were fabricated on slide glass by vacuum deposition and the application of a photolithographic technique. The micro-chamber (4 x 50 x 1 mm, volume 200 microl) was fabricated on a poly(dimethylsiloxane) (PDMS) polymer. The Pt electrode was implanted in a PDMS polymer. DMSO reductase was immobilized on a Au film electrode with bovine serum albumin (BSA)-glutaraldehyde. This sensor could determine DMSO in an unpurged aqueous solution with glucose oxidase (GOD) and catalase (CAT) for oxygen removal. The DMSO sensor showed a linear response within 1 mM DMSO with a correlation coefficient of 0.999. The detection limit was 200 microM (3sigma), and the sensitivity was 23.8 mA M(-1) cm(-2). The relative standard deviations at each concentration were within 3.6%.

摘要

构建了一种使用二甲基亚砜还原酶和薄膜电极的新型二甲基亚砜（DMSO）传感器。通过真空沉积和光刻技术在载玻片上制备金电极和银电极。在聚二甲基硅氧烷（PDMS）聚合物上制备微腔（4×50×1mm，体积200微升）。将铂电极植入PDMS聚合物中。用牛血清白蛋白（BSA）-戊二醛将二甲基亚砜还原酶固定在金膜电极上。该传感器可以在未净化的水溶液中，利用葡萄糖氧化酶（GOD）和过氧化氢酶（CAT）去除氧气来测定二甲基亚砜。该二甲基亚砜传感器在1mM二甲基亚砜范围内呈现线性响应，相关系数为0.999。检测限为200μM（3σ），灵敏度为23.8 mA M(-1) cm(-2)。各浓度下的相对标准偏差在3.6%以内。