牛嗜铬细胞中含磷酸酪氨酸的蛋白质：胰岛素样生长因子I（IGF-I）的作用。
Phosphotyrosine-containing proteins in bovine chromaffin cells: effects of insulin-like growth factor I (IGF-I).
作者信息
Cahill A L, Perlman R L
机构信息
Department of Pediatrics, University of Chicago, Illinois 60637.
出版信息
Cell Mol Neurobiol. 1991 Aug;11(4):387-95. doi: 10.1007/BF00711420.
Abstract
- Antiphosphotyrosine antibodies were used to detect phosphotyrosine-containing proteins in immunoblots of bovine chromaffin cell proteins. 2. Unstimulated cells exhibited two major phosphotyrosine-containing proteins, which had Mr's of 121,000 and 70,000. Insulin-like growth factor I (IGF-I) had little effect on the phosphotyrosine content of these two proteins but greatly increased the phosphotyrosine content of three other proteins of Mr 185,000, 170,000, and 96,000. These proteins were found predominantly in the particulate fraction of cell homogenates. 3. The effects of the IGF-I were time and concentration dependent, with maximal increases in phosphorylation occurring after 1 min of treatment with 10 nM IGF-I. Na3VO4, an inhibitor of phosphotyrosine phosphatases, potentiated the effects of IGF-I. 4. Thus, the IGF-I receptor appears to function as an IGF-I-activated protein tyrosine kinase in chromaffin cells. The tyrosine kinase activity of the IGF-I receptor presumably mediates the effects of IGF-I on chromaffin cell function.
摘要
- 抗磷酸酪氨酸抗体用于检测牛嗜铬细胞蛋白免疫印迹中含磷酸酪氨酸的蛋白。2. 未受刺激的细胞显示出两种主要的含磷酸酪氨酸蛋白，其分子量分别为121,000和70,000。胰岛素样生长因子I（IGF-I）对这两种蛋白的磷酸酪氨酸含量影响很小，但大大增加了另外三种分子量为185,000、170,000和96,000的蛋白的磷酸酪氨酸含量。这些蛋白主要存在于细胞匀浆的颗粒部分。3. IGF-I的作用具有时间和浓度依赖性，用10 nM IGF-I处理1分钟后磷酸化增加最大。磷酸酪氨酸磷酸酶抑制剂Na3VO4增强了IGF-I的作用。4. 因此，IGF-I受体似乎在嗜铬细胞中作为IGF-I激活的蛋白酪氨酸激酶发挥作用。IGF-I受体的酪氨酸激酶活性大概介导了IGF-I对嗜铬细胞功能的影响。
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