Alada A R A, Salahdeen H M, Akande O O, Idolor G O
Department of Physiology, College of Medicine, University of Ibadan, Ibadan,
Niger J Physiol Sci. 2005 Jun-Dec;20(1-2):74-8.
The study aimed to determine the influence of nitric oxide (NO) on the action of histamine and carbachol on acid secretion in the common African toad - Bufo regularis. Gastric acidity was determined by titration method. The acid secretion was determined when nitric oxide was absent following administration of NO synthase inhibitor; N-nitro-L-arginine methyl ester (L-NAME) and when nitric oxide was in excess by administration of exogenous NO donor, sodium nitroprusside (SNP). Histamine or carbachol increased acid secretion in the toad. Acid output increased from 0.32 +/- 0.04 mEq/15min to 0.56 +/- 0.08 and 0.61 +/- 0.05 mEq/15min for histamine and carbachol respectively [P < 0.05]. Pretreatment of the toad with L-NAME produced further increases in histamine (0.62 +/- 0.06 mEq/15min) or carbachol (0.74 +/- 0.06 mEq/15min) induced acid secretion respectively. SNP however, completely abolished the acid secretion stimulated by either histamine or carbachol. It was therefore concluded that nitric oxide has a negative influence on the histamine or carbachol-stimulated acid secretion in the toad - Bufo regularis.
该研究旨在确定一氧化氮（NO）对组胺和卡巴胆碱作用于普通非洲蟾蜍（Bufo regularis）胃酸分泌的影响。通过滴定法测定胃酸度。在给予NO合酶抑制剂N-硝基-L-精氨酸甲酯（L-NAME）后，当不存在一氧化氮时测定胃酸分泌；在给予外源性NO供体硝普钠（SNP）后，当一氧化氮过量时测定胃酸分泌。组胺或卡巴胆碱可增加蟾蜍的胃酸分泌。组胺和卡巴胆碱引起的胃酸分泌量分别从0.32±0.04 mEq/15min增加到0.56±0.08和0.61±0.05 mEq/15min[P<0.05]。用L-NAME预处理蟾蜍后，组胺（0.62±0.06 mEq/15min）或卡巴胆碱（0.74±0.06 mEq/15min）诱导的胃酸分泌进一步增加。然而，SNP完全消除了组胺或卡巴胆碱刺激的胃酸分泌。因此得出结论，一氧化氮对蟾蜍（Bufo regularis）组胺或卡巴胆碱刺激的胃酸分泌有负面影响。
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