[单克隆抗体13B1识别的重组白细胞介素-2抗原决定簇的定位]
[Localization of an antigenic determinant of recombinant interleukin-2, recognized by monoclonal antibody 13B1].
作者信息
Skrivelis V A, Bundulis Iu P, Paniutich A V, Voĭtenok N N, Lunev V E, Nesmeianov V A, Tsimanis A Iu, Gren E Ia
出版信息
Bioorg Khim. 1991 Jun;17(6):725-31.
PMID:1723267
Abstract
We suggest a simple approach to localization of antigenic determinants for the monoclonal antibody 13B1 raised against recombinant human interleukin-2. The approach is based on the limited trypsin proteolysis, peptide separation by the O'Farrell method and identification of the peptides, interacting with monoclonal antibodies, and comparison of the charge and length of these peptides with the corresponding values of theoretically possible peptides.
摘要
我们提出了一种简单的方法来定位针对重组人白细胞介素-2产生的单克隆抗体13B1的抗原决定簇。该方法基于有限的胰蛋白酶蛋白水解、通过奥法雷尔方法进行肽分离、鉴定与单克隆抗体相互作用的肽，并将这些肽的电荷和长度与理论上可能的肽的相应值进行比较。
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