一种用于黑曲霉孢子生长检测的抗体致敏微加工悬臂梁。

An antibody-sensitized microfabricated cantilever for the growth detection of Aspergillus niger spores.

作者信息

Nugaeva Natalia, Gfeller Karin Y, Backmann Natalia, Düggelin Marcel, Lang Hans Peter, Güntherodt Hans-Joachim, Hegner Martin

机构信息

Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland.

出版信息

Microsc Microanal. 2007 Feb;13(1):13-7. doi: 10.1017/S1431927607070067.

DOI:10.1017/S1431927607070067

PMID:17234032

Abstract

We demonstrate a new sensitive biosensor for detection of vital fungal spores of Aspergillus niger. The biosensor is based on silicon microfabricated cantilever arrays operated in dynamic mode. The change in resonance frequency of the sensor is a function of mass binding to the cantilever surface. For specific A. niger spore immobilization on the cantilever, each cantilever was individually coated with anti-Aspergillus niger polyclonal antibodies. We demonstrate the detection of single A. niger spores and their subsequent growth on the functionalized cantilever surface by online measurements of resonance frequency shifts. The new biosensor operating in humid air allows quantitative and qualitative detection of A. niger spores as well as detection of vital, functional spores in situ within approximately 4 h. The detection limit of the sensor is 103 CFU mL-1. Mass sensitivity of the cantilever sensor is approximately 53 pg Hz-1.

摘要

我们展示了一种用于检测黑曲霉有活力真菌孢子的新型灵敏生物传感器。该生物传感器基于以动态模式运行的硅微加工悬臂阵列。传感器共振频率的变化是与悬臂表面结合的质量的函数。为了将特定的黑曲霉孢子固定在悬臂上，每个悬臂都单独涂覆了抗黑曲霉多克隆抗体。我们通过在线测量共振频率偏移，证明了能够检测单个黑曲霉孢子及其在功能化悬臂表面上随后的生长情况。在潮湿空气中运行的新型生物传感器能够在大约4小时内对黑曲霉孢子进行定量和定性检测，以及原位检测有活力的、功能性的孢子。该传感器的检测限为103 CFU mL-1。悬臂传感器的质量灵敏度约为53 pg Hz-1。