培养基中的酚红强烈影响人MCF-7细胞对罗可辛的敏感性。
Phenol red in the culture medium strongly affects the susceptibility of human MCF-7 cells to roscovitine.
作者信息
Wesierska-Gadek Józefa, Schreiner Tanja, Maurer Margarita, Waringer Astrid, Ranftler Carmen
机构信息
Institute of Cancer Research, Department of Medicine I, Vienna Medical University, Vienna, Austria.
出版信息
Cell Mol Biol Lett. 2007;12(2):280-93. doi: 10.2478/s11658-007-0002-5. Epub 2007 Jan 19.
Abstract
Estrogens play an important role in the growth and terminal differentiation of the mammary gland. Prolonged exposure to estrogens seems to predispose women to breast cancer. It recently became evident that not only the intrinsic hormonal status but also external factors such as the occurrence of pharmaceuticals and chemicals with hormone activity in the environment may put women at greater risk of developing breast cancer. We focused on the interference of endocrine disruptors in breast cancer therapy. We observed that phenol red added to the culture medium strongly promoted the cell proliferation and cell cycle progression of human cells expressing the estrogen receptor, and affected their susceptibility to chemotherapy.
摘要
雌激素在乳腺的生长和终末分化中起重要作用。长期暴露于雌激素似乎会使女性易患乳腺癌。最近变得明显的是，不仅内在激素状态，而且环境中具有激素活性的药物和化学物质的出现等外部因素也可能使女性患乳腺癌的风险更高。我们专注于内分泌干扰物在乳腺癌治疗中的干扰作用。我们观察到，添加到培养基中的酚红强烈促进了表达雌激素受体的人类细胞的细胞增殖和细胞周期进程，并影响了它们对化疗的敏感性。
相似文献
1
Phenol red in the culture medium strongly affects the susceptibility of human MCF-7 cells to roscovitine.
Cell Mol Biol Lett. 2007;12(2):280-93. doi: 10.2478/s11658-007-0002-5. Epub 2007 Jan 19.
2
Phenol red reduces ROSC mediated cell cycle arrest and apoptosis in human MCF-7 cells.
J Cell Biochem. 2006 Aug 15;98(6):1367-79. doi: 10.1002/jcb.20960.
3
Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500. doi: 10.1073/pnas.83.8.2496.
4
5
Roscovitine, a selective CDK inhibitor, reduces the basal and estrogen-induced phosphorylation of ER-α in human ER-positive breast cancer cells.
J Cell Biochem. 2011 Mar;112(3):761-72. doi: 10.1002/jcb.23004.
6
Resveratrol modulates roscovitine-mediated cell cycle arrest of human MCF-7 breast cancer cells.
Food Chem Toxicol. 2008 Apr;46(4):1327-33. doi: 10.1016/j.fct.2007.09.004. Epub 2007 Sep 11.
7
pH-dependent cytotoxicity of contaminants of phenol red for MCF-7 breast cancer cells.
Endocrinology. 1991 Dec;129(6):3321-30. doi: 10.1210/endo-129-6-3321.
8
Estrogenic activity of phenol red.
Mol Cell Endocrinol. 1988 Jun;57(3):169-78. doi: 10.1016/0303-7207(88)90072-x.
9
Roscovitine differentially affects asynchronously growing and synchronized human MCF-7 breast cancer cells.
Ann N Y Acad Sci. 2009 Aug;1171:250-6. doi: 10.1111/j.1749-6632.2009.04717.x.
10
Interaction of phenol red with estrogenic and antiestrogenic action on growth of human breast cancer cells ZR-75-1 and T-47-D.
Cancer Res. 1988 Jul 1;48(13):3693-7.
引用本文的文献
1
SIRT1/DNMT3B-mediated epigenetic gene silencing in response to phytoestrogens in mammary epithelial cells.
Epigenetics. 2025 Dec;20(1):2473770. doi: 10.1080/15592294.2025.2473770. Epub 2025 Mar 3.
2
Recommendations for the Application of Sex and Gender Medicine in Preclinical, Epidemiological and Clinical Research.
J Pers Med. 2024 Aug 27;14(9):908. doi: 10.3390/jpm14090908.
3
Optimized LC-MS/MS methods for quantifying antibody-drug conjugate payloads in cell culture media containing phenol red.
Bioanalysis. 2024;16(12):575-585. doi: 10.1080/17576180.2024.2349422. Epub 2024 Jun 11.
4
Biological Activity of Oleanolic Acid Derivatives HIMOXOL and Br-HIMOLID in Breast Cancer Cells Is Mediated by ER and EGFR.
Int J Mol Sci. 2023 Mar 7;24(6):5099. doi: 10.3390/ijms24065099.
5
Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells.
Methods Mol Biol. 2022;2418:313-343. doi: 10.1007/978-1-0716-1920-9_18.
6
Estrogen Plays a Crucial Role in Rab9-Dependent Mitochondrial Autophagy, Delaying Arterial Senescence.
J Am Heart Assoc. 2021 Apr 6;10(7):e019310. doi: 10.1161/JAHA.120.019310. Epub 2021 Mar 15.
7
Sirt3 Exerts Its Tumor-Suppressive Role by Increasing p53 and Attenuating Response to Estrogen in MCF-7 Cells.
Antioxidants (Basel). 2020 Apr 1;9(4):294. doi: 10.3390/antiox9040294.
8
Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells.
Methods Mol Biol. 2016;1366:373-393. doi: 10.1007/978-1-4939-3127-9_30.
9
Profiling of estrogen-regulated microRNAs in breast cancer cells.
J Vis Exp. 2014 Feb 21(84):e51285. doi: 10.3791/51285.
10
U-shape suppressive effect of phenol red on the epileptiform burst activity via activation of estrogen receptors in primary hippocampal culture.
PLoS One. 2013;8(4):e60189. doi: 10.1371/journal.pone.0060189. Epub 2013 Apr 1.
本文引用的文献
1
Phenol red reduces ROSC mediated cell cycle arrest and apoptosis in human MCF-7 cells.
J Cell Biochem. 2006 Aug 15;98(6):1367-79. doi: 10.1002/jcb.20960.
2
Anti-estrogenic effects of conjugated linoleic acid through modulation of estrogen receptor phosphorylation.
Breast Cancer Res Treat. 2005 Nov;94(2):161-9. doi: 10.1007/s10549-005-6942-4.
3
Adaptation of estrogen-regulated genes in long-term estradiol deprived MCF-7 breast cancer cells.
Breast Cancer Res Treat. 2005 Dec;94(3):213-23. doi: 10.1007/s10549-005-5776-4.
4
A new multiplex assay allowing simultaneous detection of the inhibition of cell proliferation and induction of cell death.
J Cell Biochem. 2005 Sep 1;96(1):1-7. doi: 10.1002/jcb.20531.
5
Roscovitine-induced up-regulation of p53AIP1 protein precedes the onset of apoptosis in human MCF-7 breast cancer cells.
Mol Cancer Ther. 2005 Jan;4(1):113-24.
6
Increased susceptibility of poly(ADP-ribose) polymerase-1 knockout cells to antitumor triazoloacridone C-1305 is associated with permanent G2 cell cycle arrest.
Cancer Res. 2004 Jul 1;64(13):4487-97. doi: 10.1158/0008-5472.CAN-03-3410.
7
Dual action of cyclin-dependent kinase inhibitors: induction of cell cycle arrest and apoptosis. A comparison of the effects exerted by roscovitine and cisplatin.
Pol J Pharmacol. 2003 Sep-Oct;55(5):895-902.
8
Apoptosis - the p53 network.
J Cell Sci. 2003 Oct 15;116(Pt 20):4077-85. doi: 10.1242/jcs.00739.
9
Rapid onset of nucleolar disintegration preceding cell cycle arrest in roscovitine-induced apoptosis of human MCF-7 breast cancer cells.
Int J Cancer. 2003 Sep 10;106(4):486-495. doi: 10.1002/ijc.11290.
10
Enhanced estrogen receptor (ER) alpha, ERBB2, and MAPK signal transduction pathways operate during the adaptation of MCF-7 cells to long term estrogen deprivation.
J Biol Chem. 2003 Aug 15;278(33):30458-68. doi: 10.1074/jbc.M305226200. Epub 2003 May 29.
Zotero 插件